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Human SIRP beta 1/CD172b Antibody

Catalog #: MAB20961 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB20961-100
MAB20961-SP
Detection of SIRP-beta in Human Peripheral Blood Granulocytes by Flow Cytometry.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SIRP beta 1/CD172b Antibody Summary

Species Reactivity
Human
Specificity
Detects human SIRP beta 1/CD172b in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 308908
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SIRP beta 1/CD172b
Gly26-Ala369 (Arg53His and Ala363Pro)
Accession # O00241.4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of SIRP-beta antibody in Human Peripheral Blood Granulocytes antibody by Flow Cytometry. View Larger

Detection of SIRP-beta in Human Peripheral Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human SIRP-beta Monoclonal Antibody (Catalog # MAB20961, filled histogram) or Mouse IgG1 Isotype Control Antibody (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry SIRP beta 1/CD172b antibody in U937 human histiocytic lymphoma cell line by Immunocytochemistry (ICC). View Larger

SIRP beta 1/CD172b in U937 human histiocytic lymphoma cell line. SIRP beta 1/CD172b was detected in immersion fixed U937 human histiocytic lymphoma cell line (left panel; positive staining) and Daudi human Burkitt's lymphoma cell line (right panel; negative staining) using Mouse Anti-Human SIRP beta 1/CD172b Monoclonal Antibody (Catalog # MAB20961) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIRP beta 1/CD172b

SIRP beta 1 is a type I transmembrane protein belonging to the SIRP family within the Ig superfamily. Members of this family are characterized by an extracellular region containing a V-set Ig domain containing a J-like sequence and two C1-set Ig domains. Unlike SIRP alpha that has cytoplasmic ITIM domains, SIRP beta 1 possesses positively charged residues that allow association with ITAM motif containing adaptor molecules. SIRP beta 1 is expressed on cells of monocyte, macrophage or dendritic lineages.

Long Name
Signal-regulatory Protein beta 1
Entrez Gene IDs
10326 (Human); 102139733 (Cynomolgus Monkey)
Alternate Names
CD172 antigen-like family member B; CD172b antigen; CD172b; DKFZp686A05192; FLJ26614; signal-regulatory protein beta 1; signal-regulatory protein beta-1; SIRP beta 1; SIRPB1; SIRP-beta-1 isoform 3; SIRP-BETA-1

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