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Human SIRP beta 1/gamma Antibody

Catalog #: MAB2096 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2096
MAB2096-SP
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SIRP beta 1/gamma Antibody Summary

Species Reactivity
Human
Specificity
Detects human SIRP beta 1 and human SIRP gamma in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2A Clone # 308906
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SIRP beta 1
Gly26-Ala369 (Arg53His and Ala363Pro)
Accession # O00241.4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human SIRP beta 1 and Recombinant Human SIRP gamma
Flow Cytometry
2.5 µg/106 cells
Human whole blood
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIRP beta 1/gamma

SIRP beta 1 is a type I transmembrane protein belonging to the SIRP family within the Ig superfamily. Members of this family are characterized by an extracellular region containing a V-set Ig domain containing a J-like sequence and two C1-set Ig domains. Unlike SIRP alpha that has cytoplasmic ITIM domains, SIRP beta 1 possesses positively charged residues that allow association with ITAM motif containing adaptor molecules. SIRP beta 1 is expressed on cells of monocyte, macrophage or dendritic lineages.

Long Name
Signal-regulatory Protein beta 1/gamma
Entrez Gene IDs
10326 (Human)
Alternate Names
CD172 antigen-like family member B; CD172b; signal-regulatory protein beta-1; SIRP beta 1/gamma; SIRP-BETA-1

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