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Human Siglec-5/Siglec-14 Antibody

Catalog #: MAB10721
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB10721
MAB10721-SP
Detection of Siglec‑5/14 in Human Monocytes by Flow Cytometry.
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Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Siglec-5/Siglec-14 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Siglec‑5/Siglec‑14 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) Siglec-14 is observed and no cross-reactivity with rhSiglec-3, rhSiglec-7, or rhSiglec-9 is observed.
Source
Monoclonal Mouse IgG1 Clone # 194128
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec‑5/Siglec‑14
Lys18-Thr434
Accession # O15389
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human Siglec-5 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Siglec‑5/Siglec‑14 Biotinylated Antibody (Catalog # BAM10722)

Standard: Recombinant Human Siglec-5 Fc Chimera Protein, CF (Catalog # 1072-SL)

Neutralization
Measured by its ability to neutralize Siglec‑5-mediated adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The Neutralization Dose (ND50) is typically 0.04-0.2 µg/mL in the presence of 5 µg/mL Recombinant Human Siglec‑5 Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Siglec‑5/14 antibody in Human Monocytes antibody by Flow Cytometry. View Larger

Detection of Siglec‑5/14 in Human Monocytes by Flow Cytometry. Human blood derived monocytes were stained with Mouse Anti-Human Siglec-5/14 Monoclonal Antibody (Catalog # MAB10721, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Neutralization Cell Adhesion Mediated by Siglec‑5 and Neutralization by Human Siglec‑5/14 Antibody. View Larger

Cell Adhesion Mediated by Siglec‑5 and Neutralization by Human Siglec‑5/14 Antibody. Recombinant Human Siglec-5 Fc Chimera (Catalog # 1072-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Siglec-5 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Siglec-5/14 Mono-clonal Antibody (Catalog # MAB10721). The ND50 is typically 0.04-0.2 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-5/Siglec-14

Siglecs (sialic acid binding Ig-like lectins) are a subgroup of the immunoglobulin superfamily that interact with sialic acids in glycoproteins and glycolipids. Siglec-5 binds to alpha-2, 3- and alpha-2, 6-linked sialic acid equally. It occurs as a disulfide-linked dimer of approximately 140 kDa with the highest expression levels in hematopoietic tissues. Siglec-5 has an inhibitory motif within its cytoplasmic domain. Siglec-14 is an activating receptor that shares 99.5% aa sequence identity with Siglec-5 through the first two extracellular Ig domains and displays a similar glycan binding preference.

Long Name
Sialic Acid Binding Ig-like Lectin 5
Entrez Gene IDs
8778 (Human)
Alternate Names
CD170;CD33L2;OBBP2;OB-BP2;Sialic acid-binding Ig-like lectin 5;SIGLEC5;SIGLEC-5; Siglec-5/Siglec-14

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Citations for Human Siglec-5/Siglec-14 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Early Identification of Bronchopulmonary Dysplasia Using Novel Biomarkers by Proteomic Screening
    Authors: Kai Förster, Steffen Sass, Harald Ehrhardt, Daphne S. Mous, Robbert J. Rottier, Prajakta Oak et al.
    American Journal of Respiratory and Critical Care Medicine
  2. The Paired Siglecs in Brain Tumours Therapy: The Immunomodulatory Effect of Dexamethasone and Temozolomide in Human Glioma In Vitro Model
    Authors: P Wielgat, N Wawrusiewi, R Czarnomysy, K Rogowski, K Bielawski, H Car
    International Journal of Molecular Sciences, 2021-02-11;22(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Identification of lipophilic ligands of Siglec5 and 14 that modulate innate immune responses
    Authors: R Suematsu, T Miyamoto, S Saijo, S Yamasaki, Y Tada, H Yoshida, Y Miyake
    J. Biol. Chem., 2019-09-24;0(0):.
    Species: Fungus
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Western Blot
  4. Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
    Authors: PJ Huang, PY Low, I Wang, SD Hsu, T Angata
    J. Biol. Chem., 2018-10-30;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  5. Siglec-1 is a novel dendritic cell receptor that mediates HIV-1 trans-infection through recognition of viral membrane gangliosides.
    Authors: Izquierdo-Useros N, Lorizate M, Puertas M, Rodriguez-Plata M, Zangger N, Erikson E, Pino M, Erkizia I, Glass B, Clotet B, Keppler O, Telenti A, Krausslich H, Martinez-Picado J
    PLoS Biol, 2012-12-18;10(12):e1001448.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Sialic acid residues play a pivotal role in alpha(1)-acid glycoprotein (AGP)-induced generation of reactive oxygen species in chemotactic peptide pre-activated neutrophil granulocytes.
    Authors: Gunnarsson P, Fornander L, Pahlsson P, Grenegard M
    Inflamm. Res., 2009-08-11;59(2):89-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Group B Streptococcus suppression of phagocyte functions by protein-mediated engagement of human Siglec-5.
    Authors: Carlin AF, Chang YC, Areschoug T, Lindahl G, Hurtado-Ziola N, King CC, Varki A, Nizet V
    J. Exp. Med., 2009-07-13;206(8):1691-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates.
    Authors: Angata T, Hayakawa T, Yamanaka M, Varki A, Nakamura M
    FASEB J., 2006-10-01;20(12):1964-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity
    Sci Rep, 2016-11-28;6(0):37953.
  10. Siglec-14 Enhances NLRP3-Inflammasome Activation in Macrophages
    Authors: Chih-Ming Tsai, Angelica M. Riestra, Syed Raza Ali, Jerry J. Fong, Janet Z. Liu, Gillian Hughes et al.
    Journal of Innate Immunity

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