Human SGPL1 Antibody Summary
Lys342-His568
Accession # O95470
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human SGPL1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, HepG2 human hepatocellular carcinoma cell line, HT-29 human colon adenocarcinoma cell line, and HT-29 tumor tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SGPL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5535) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SGPL1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Detection of Human SGPL1 by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SGPL1 at approximately 60 kDa (as indicated) using 50 µg/mL of Goat Anti-Human SGPL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5535) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SGPL1
SGPL1 (Sphingosine-1-phosphate lyase; also hSPL) is a 60‑63 kDa member of the SGPL1 subfamily, group II decarboxylase family of enzymes. It is very widely expressed, and particularly concentrated in cells undergoing rapid turnover. SGPL1 is found in the ER, and cleaves phosphorylated sphingoid bases into fatty aldehydes and phosphoethanolamine. This regulates a diversity of cellular processes such as apoptosis and T cell migration. Human SGPL1 is 568 amino acids (aa) in length. It is a type III transmembrane protein (luminal N-terminus with no signal sequence) that contains a 507 aa cytoplasmic domain (aa 62‑568). This domain contains a vitamin B6 binding site (aa 344‑364) that is part of a large catalytic region (aa 116‑506). Over aa 342‑568, human SGPL1 shares 86% aa identity with mouse SGPL1.
Product Datasheets
Citations for Human SGPL1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
14
Citations: Showing 1 - 10
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Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction
Authors: A. Maharaj, J. Williams, T. Bradshaw, T. Güran, D. Braslavsky, J. Casas et al.
The Journal of Steroid Biochemistry and Molecular Biology
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Insights From Long-term Follow-up of a Girl With Adrenal Insufficiency and Sphingosine-1-Phosphate Lyase Deficiency
Authors: Avinaash Maharaj, Tülay Güran, Federica Buonocore, John C Achermann, Louise Metherell, Rathi Prasad et al.
Journal of the Endocrine Society
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A Sphingosine-1-Phosphate Lyase Mutation Associated With Congenital Nephrotic Syndrome and Multiple Endocrinopathy
Authors: Avinaash Maharaj, Demetria Theodorou, Indraneel (Indi) Banerjee, Louise A. Metherell, Rathi Prasad, Dean Wallace
Frontiers in Pediatrics
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AAV-SPL 2.0, a Modified Adeno-Associated Virus Gene Therapy Agent for the Treatment of Sphingosine Phosphate Lyase Insufficiency Syndrome
Authors: Khan, R;Oskouian, B;Lee, JY;Hodgin, JB;Yang, Y;Tassew, G;Saba, JD;
International journal of molecular sciences
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Sphingosine Phosphate Lyase Is Upregulated in Duchenne Muscular Dystrophy, and Its Inhibition Early in Life Attenuates Inflammation and Dystrophy in Mdx Mice
Authors: AS De la Garz, SA Moore, J Zamora-Pin, EP Hoffman, K Mistry, A Kumar, JB Strober, P Zhao, JH Suh, JD Saba
International Journal of Molecular Sciences, 2022-07-08;23(14):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Efficacy of AAV9-mediated SGPL1 gene transfer in a mouse model of S1P lyase insufficiency syndrome
Authors: P Zhao, GB Tassew, JY Lee, B Oskouian, DP Muñoz, JB Hodgin, GL Watson, F Tang, JY Wang, J Luo, Y Yang, SM King, RM Krauss, N Keller, JD Saba
JCI Insight, 2021-04-22;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Heterogenous Nuclear Ribonucleoprotein H1 Promotes Colorectal Cancer Progression through the Stabilization of mRNA of Sphingosine-1-Phosphate Lyase 1
Authors: K Takahashi, M Fujiya, H Konishi, Y Murakami, T Iwama, T Sasaki, T Kunogi, A Sakatani, K Ando, N Ueno, S Kashima, K Moriichi, H Tanabe, T Okumura
Int J Mol Sci, 2020-06-25;21(12):.
Species: Human
Sample Types: Cell Culture Lysates
Applications: Western Blot -
Disarranged Sphingolipid Metabolism From Sphingosine-1-Phosphate Lyase Deficiency Leads to Congenital Nephrotic Syndrome
Authors: VA Taylor, HK Stone, MP Schuh, X Zhao, KD Setchell, E Erkan
Kidney Int Rep, 2019-08-07;4(12):1763-1769.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Nicotinamide phosphoribosyltransferase is a molecular target of potent anticancer agents identified from phenotype-based drug screening
Authors: D Yamaguchi, T Imaizumi, K Yagi, Y Matsumoto, T Nakashima, A Hirose, N Kashima, Y Nosaka, T Hamada, K Okawa, Y Nishiya, K Kubo
Sci Rep, 2019-05-23;9(1):7742.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Mutations in sphingosine-1-phosphate lyase cause nephrosis with ichthyosis and adrenal insufficiency
Authors: S Lovric, S Goncalves, HY Gee, B Oskouian, H Srinivas, WI Choi, S Shril, S Ashraf, W Tan, J Rao, M Airik, D Schapiro, DA Braun, CE Sadowski, E Widmeier, T Jobst-Schw, JM Schmidt, V Girik, G Capitani, JH Suh, N Lachaussée, C Arrondel, J Patat, O Gribouval, M Furlano, O Boyer, A Schmitt, V Vuiblet, S Hashmi, R Wilcken, FP Bernier, AM Innes, JS Parboosing, RE Lamont, JP Midgley, N Wright, J Majewski, M Zenker, F Schaefer, N Kuss, J Greil, T Giese, K Schwarz, V Catheline, D Schanze, I Franke, Y Sznajer, AS Truant, B Adams, J Désir, R Biemann, Y Pei, E Ars, N Lloberas, A Madrid, VR Dharnidhar, AM Connolly, MC Willing, MA Cooper, RP Lifton, M Simons, H Riezman, C Antignac, JD Saba, F Hildebrand
J. Clin. Invest., 2017-02-06;127(3):912-928.
Species: Mouse, Rat
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
Expression of bioactive lysophospholipids and processing enzymes in the vitreous from patients with proliferative diabetic retinopathy.
Authors: Abu El-Asrar, Ahmed M, Nawaz, Mohd Imt, Mohammad, Ghulam, Siddiquei, Mohammad, Alam, Kaiser, Mousa, Ahmed, Opdenakker, Ghislain
Lipids Health Dis, 2014-12-11;13(0):187.
Species: Human
Sample Types: Vitreous Humor
Applications: Western Blot -
Sphingosine-1-phosphate (S1P) displays sustained S1P1 receptor agonism and signaling through S1P lyase-dependent receptor recycling.
Authors: Gatfield J, Monnier L, Studer R, Bolli M, Steiner B, Nayler O
Cell Signal, 2014-04-02;26(7):1576-88.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Increased expression of sphingosine kinase in the amnion during labor.
Authors: Erkhembaatar L, Kotani T, Sumigama S, Tsuda H, Mano Y, Hua L, Hasegawa Y, Wang J, Sugiyama C, Nakahara T, Iwase A, Kikkawa F
Placenta, 2013-02-22;34(4):353-9.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-P, Western Blot -
ER Stress-Induced Sphingosine-1-Phosphate Lyase Phosphorylation Potentiates the Mitochondrial Unfolded Protein Response
Authors: Asli D. Yildirim, Mevlut Citir, Asli E. Dogan, Zehra Veli, Zehra Yildirim, Ozlem Tufanli et al.
Journal of Lipid Research
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