Human SF-1/NR5A1 Antibody

Catalog #: MAB107241 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB107241-100
MAB107241-SP

Detection of Human SF‑1/NR5A1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SF-1/NR5A1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SF‑1/NR5A1 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1036724

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human SF‑1/NR5A1 synthetic peptide
Accession # Q13286

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

Human adrenal gland and human spleen

Immunocytochemistry

8-25 µg/mL

Immersion fixed HepG2 human hepatocellular carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SF‑1/NR5A1 by Western Blot. Western blot shows lysates of human adrenal gland and human spleen. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SF‑1/NR5A1 Monoclonal Antibody (Catalog # MAB107241) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for SF‑1/NR5A1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunocytochemistry

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SF‑1/NR5A1 in HepG2 Human Cell Line. SF‑1/NR5A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining) and K562 human chronic myelogenous leukemia cell line (negative control) using Mouse Anti-Human SF‑1/NR5A1 Monoclonal Antibody (Catalog # MAB107241) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SF-1/NR5A1

SF-1 (also NR5A1 and STF1) is a transcriptional activator essential for sexual differentiation and formation of the primary steroidogenic tissues. Mutations in the NR5A1 gene result in multiple diseases ranging from infertility (SFPG8), premature ovarian failure (POF7), adrenal insufficiency (AINR), female to male sex reversal (SRXX4) and male to female sex reversal (SRXY3).

Long Name

Steroidogenic Factor 1

Entrez Gene IDs

2516 (Human)

Alternate Names

AD4BP; AD4BPSTF-1; Adrenal 4-binding protein; ELP; FTZ1adrenal 4 binding protein; FTZF1; FTZF1nuclear receptor AdBP4; Fushi tarazu factor homolog 1; NR5A1; Nuclear receptor subfamily 5 group A member 1; nuclear receptor subfamily 5, group A, member 1; SF1; SF-1; SF-1POF7; SF1steroidogenic factor 1; Steroid hormone receptor Ad4BP; steroidogenic factor-1