Human Serpin G1/C1 Inhibitor ELISA Kit - Quantikine

Catalog #: DSGC10 Datasheet / COA / SDS

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Serpin G1/C1 Inhibitor Quantikine ELISA Kit Summary

Assay Length

4.5 hours

Sensitivity

25.6 pg/mL

Assay Range

62.5 - 2,000 pg/mL (Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)

Specificity

Natural human Serpin G1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine™ Human Serpin G1/C1 Inhibitor Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Serpin G1 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains human Serpin G1 purified from plasma and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Serpin G1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Serpin G1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	206	634	1209	212	6.3	1191
Standard Deviation	10.4	24.7	56.5	13.7	31.7	53.4
CV%	5	3.9	4.7	6.5	5.3	4.5

Recovery

The recovery of human Serpin G1 spiked to levels throughout the range of the assay in cell culture media was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	100	90-110

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Serpin G1 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Serpin G1/C1 Inhibitor

The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.

Entrez Gene IDs:

710 (Human)

Alternate Names:

C1 Inhibitor; C1IN; C1INH; C1NH; HAE1; HAE2; Serpin G1; SERPING1 serpin peptidase inhibitor, clade G (C1 inhibitor), member 1