Human sE-Selectin/CD62E Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Serum, Heparin Plasma, Citrate Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (ng/mL) | 1.6 | 2.9 | 4.7 | 1.5 | 2.9 | 4.6 |
Standard Deviation | 0.11 | 0.17 | 0.24 | 0.11 | 0.25 | 0.35 |
CV% | 6.6 | 5.7 | 5.2 | 7.3 | 8.7 | 7.7 |
Recovery
The recovery of sE-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 103 | 98-110 |
Citrate Plasma (n=4) | 96 | 85-107 |
Heparin Plasma (n=4) | 94 | 85-107 |
Serum (n=4) | 96 | 85-106 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: E-Selectin/CD62E
The Selectin family is comprised of three members, E-Selectin, L-Selectin, and P-Selectin. E-Selectin [endothelial leukocyte adhesion molecule-1 (ELAM-1), CD62E] is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. The human and rat proteins share approximately 67% amino acid sequence identity. The mouse and rat proteins share approximately 78% amino acid sequence identity. L-Selectin (Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L) is expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. Human and mouse L-Selectin share 76% amino acid sequence identity. Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is expressed by activated platelets and endothelial cells. The extracellular domains of human and mouse P-Selectin share approximately 73% amino acid sequence identity.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human sE-Selectin/CD62E Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Endothelial Dysfunction Biomarkers and CKD Incidence in the REGARDS Cohort
Authors: Short, SAP;Wilkinson, K;Long, DL;Crews, DC;Gutierrez, OM;Irvin, MR;Wheeler, M;Cushman, M;Cheung, KL;
Kidney international reports
Species: Human
Sample Types: Plasma
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Association of circulating biomarkers with illness severity measures differentiates myalgic encephalomyelitis/chronic fatigue syndrome and post-COVID-19 condition: a prospective pilot cohort study
Authors: Domingo, JC;Battistini, F;Cordobilla, B;Zaragozá, MC;Sanmartin-Sentañes, R;Alegre-Martin, J;Cambras, T;Castro-Marrero, J;
Journal of translational medicine
Species: Human
Sample Types: Plasma
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Circulating Soluble EPCR Levels Are Reduced in Patients with Ischemic Peripheral Artery Disease and Associated with Markers of Endothelial and Vascular Function
Authors: Krug, J;Bochenek, ML;Gogiraju, R;Laubert-Reh, D;Lackner, KJ;Münzel, T;Wild, PS;Espinola-Klein, C;Schäfer, K;
Biomedicines
Species: Human
Sample Types: Plasma
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Lung Ultrasound Findings and Endothelial Perturbation in a COVID-19 Low-Intensity Care Unit
Authors: R Gualtierot, F Tafuri, R Rossio, M Rota, P Bucciarell, B Ferrari, A Giachi, C Suffritti, M Cugno, F Peyvandi, On Behalf
Journal of Clinical Medicine, 2022-09-15;11(18):.
Species: Human
Sample Types: Plasma
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Plasma Endothelial and Oxidative Stress Biomarkers Associated with Late Mortality in Hospitalized COVID-19 Patients
Authors: A Orea-Tejad, C Sánchez-Mo, OG Aztatzi-Ag, MP Sierra-Var, D González-I, Y Debray-Gar, MS Ortega-Rom, C Keirns-Dav, L Cornejo-Co, J Aguilar-Me
Journal of Clinical Medicine, 2022-07-07;11(14):.
Species: Human
Sample Types: Plasma
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Vascular and blood-brain barrier-related changes underlie stress responses and resilience in female mice and depression in human tissue
Authors: L Dion-Alber, A Cadoret, E Doney, FN Kaufmann, KA Dudek, B Daigle, LF Parise, F Cathomas, N Samba, N Hudson, M Lebel, Signature, M Campbell, G Turecki, N Mechawar, C Menard
Nature Communications, 2022-01-10;13(1):164.
Species: Human
Sample Types: Serum
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Effect of the Renin-Angiotensin-Aldosterone System Reactivity on Endothelial Function and Modulative Role of Valsartan in Male Subjects with Essential Hypertension
Authors: J Jasiczek, M Trocha, A Derkacz, E Szahidewic, A Doroszko
Journal of Clinical Medicine, 2021-12-13;10(24):.
Species: Human
Sample Types: Plasma
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Differences in the Biomarker Profile of De Novo Acute Heart Failure versus Decompensation of Chronic Heart Failure
Authors: S Nawrocka-M, J Biegus, M Hurkacz, M Guzik, M Rosiek-Bie, EA Jankowska, P Ponikowski, R Zymli?ski
Biomolecules, 2021-11-16;11(11):.
Species: Human
Sample Types: Plasma
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Tahini consumption affects blood pressure and endothelial function in healthy males
Authors: EI Sakketou, GK Baxevanis, NK Tentolouri, GD Konstanton, VT Karathanos, GA Fragkiadak, PT Kanellos
Journal of human hypertension, 2021-10-27;0(0):.
Species: Human
Sample Types: Serum
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Disturbed Lipid Metabolism in Diabetic Patients with Manifest Coronary Artery Disease Is Associated with Enhanced Inflammation
Authors: K Buschmann, Y Gramlich, R Chaban, M Oelze, U Hink, T Münzel, H Treede, A Daiber, GD Duerr
International Journal of Environmental Research and Public Health, 2021-10-17;18(20):.
Species: Human
Sample Types: Serum
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Relationship of low molecular weight fluorophore levels with clinical factors and fenofibrate effects in adults with type 2 diabetes
Authors: AS Januszewsk, D Chen, RS Scott, RL O'Connell, NR Aryal, DR Sullivan, GF Watts, MR Taskinen, PJ Barter, JD Best, RJ Simes, AC Keech, AJ Jenkins
Scientific Reports, 2021-09-21;11(1):18708.
Species: Human
Sample Types: Serum
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The Effect of Caloric Restriction with and without n-3 PUFA Supplementation on Bone Turnover Markers in Blood of Subjects with Abdominal Obesity: A Randomized Placebo-Controlled Trial
Authors: U Razny, J Goralska, PC Calder, A Gruca, CE Childs, M Kapusta, K Slowinska-, A Dembinska-, B Solnica, M Malczewska
Nutrients, 2021-09-02;13(9):.
Species: Human
Sample Types: Serum
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Endothelial glycocalyx degradation and disease severity in Plasmodium vivax and Plasmodium knowlesi malaria
Authors: BE Barber, MJ Grigg, KA Piera, Y Chen, T William, JB Weinberg, TW Yeo, NM Anstey
Scientific Reports, 2021-05-07;11(1):9741.
Species: Human
Sample Types: Plasma
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Functional disability is related to serum chemerin levels in rheumatoid arthritis
Authors: ML Vazquez-Vi, JI Gamez-Nava, AM Saldaña-Cr, A Celis, EN Sanchez-Ro, EE Perez-Guer, M Ramirez-Vi, CA Nava-Valdi, B Contreras-, JC Vasquez-Ji, JM Ponce-Guar, AK Barocio-Ra, S Cerpa-Cruz, MF Alcaraz-Lo, L Gonzalez-L
Scientific Reports, 2021-04-16;11(1):8360.
Species: Human
Sample Types: Serum
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Resveratrol and exercise combined to treat functional limitations in late life: A pilot randomized controlled trial
Authors: SA Harper, JR Bassler, S Peramsetty, Y Yang, LM Roberts, D Drummer, RT Mankowski, C Leeuwenbur, K Ricart, RP Patel, MM Bamman, SD Anton, BC Jaeger, TW Buford
Exp Gerontol, 2020-10-15;0(0):111111.
Species: Human
Sample Types: Plasma
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Markers of endothelial cell activation and neutrophil extracellular traps are elevated in immune thrombocytopenia but are not enhanced by thrombopoietin receptor agonists
Authors: L Garabet, CE Henriksson, ML Lozano, W Ghanima, J Bussel, E Brodin, MP Fernández-, C Martínez, R González-C, MC Mowinckel, PM Sandset
Thromb. Res., 2019-11-30;185(0):119-124.
Species: Human
Sample Types: Serum
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High social strain and physical health: Examining the roles of anxious arousal, body mass index, and inflammation
Authors: JE Guevara, KW Murdock
Psychoneuroendocrinology, 2019-04-05;106(0):155-160.
Species: Human
Sample Types: Serum
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Lp-PLA2, scavenger receptor class B type I gene (SCARB1) rs10846744 variant, and cardiovascular disease
Authors: A Manichaiku, XQ Wang, L Li, J Erdmann, G Lettre, JC Bis, D Waterworth, M Cushman, NS Jenny, WS Post, W Palmas, MY Tsai, L Wallentin, H White, H Schunkert, CJ O'Donnell, DM Herrington, SS Rich, ML O'Donoghue, A Rodriguez
PLoS ONE, 2018-10-05;13(10):e0204352.
Species: Human
Sample Types: Plasma
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How Could We Influence Systemic Inflammation in Allergic Rhinitis? The Role of H1 Antihistamines
Authors: IA Muntean, IC Bocsan, N Miron, AD Buzoianu, D Deleanu
Oxid Med Cell Longev, 2018-06-12;2018(0):3718437.
Species: Human
Sample Types: Plasma
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Targeted endothelial gene deletion of Triggering Receptor Expressed on Myeloid cells-1 protects mice during septic shock
Authors: L Jolly, K Carrasco, M Derive, J Lemarié, A Boufenzer, S Gibot
Cardiovasc. Res., 2018-05-01;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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A Pecan-Rich Diet Improves Cardiometabolic Risk Factors in Overweight and Obese Adults: A Randomized Controlled Trial
Authors: DL McKay, M Eliasziw, CYO Chen, JB Blumberg
Nutrients, 2018-03-11;10(3):.
Species: Human
Sample Types: Serum
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Acute effect of different concentrations of cayenne pepper cataplasm on sensory-motor functions and serum levels of inflammation-related biomarkers in healthy subjects
Authors: N Sarabon, S Löfler, J Cvecka, W Hübl, S Zampieri
Eur J Transl Myol, 2018-03-01;28(1):7333.
Species: Human
Sample Types: Serum
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Nesfatin-1 and visfatin expression is associated with reduced atherosclerotic disease risk in patients with rheumatoid arthritis
Authors: C Robinson, L Tsang, A Solomon, AJ Woodiwiss, S Gunter, M Mer, HC Hsu, M Gomes, GR Norton, AME Millen, PH Dessein
Peptides, 2018-02-21;102(0):31-37.
Species: Human
Sample Types: Plasma
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Association of circulating CTRP9 with soluble adhesion molecules and inflammatory markers in patients with type 2 diabetes mellitus and coronary artery disease
Authors: N Moradi, R Fadaei, S Emamgholip, E Kazemian, G Panahi, S Vahedi, L Saed, S Fallah
PLoS ONE, 2018-01-30;13(1):e0192159.
Species: Human
Sample Types: Serum
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Plasma irisin is elevated in type 2 diabetes and is associated with increased E-selectin levels
Authors: KS Rana, C Pararasa, I Afzal, DA Nagel, EJ Hill, CJ Bailey, HR Griffiths, I Kyrou, HS Randeva, S Bellary, JE Brown
Cardiovasc Diabetol, 2017-11-09;16(1):147.
Species: Human
Sample Types: Plasma
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Effect of palm-based tocotrienols and tocopherol mixture supplementation on platelet aggregation in subjects with metabolic syndrome: a randomised controlled trial
Authors: YL Gan, JY Fu, OM Lai, BH Chew, KH Yuen, KT Teng, K Nesaretnam, KR Selvaduray, P Meganathan
Sci Rep, 2017-09-14;7(1):11542.
Species: Human
Sample Types: Plasma
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Evaluation of Association Between the Serum Levels of MMP-9 and MMP-9/TIMPs With Soluble Forms of Selectins and Itching Induced by Sulfur Mustard
Authors: N Askari, T Ghazanfari, MM Naghizadeh, A Moin, A Khamesipou, S Pourfarzam, ZM Hassan
Iran J Pathol, 2017-01-27;12(3):257-264.
Species: Human
Sample Types: Serum
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Cardiovascular diseases-related GNB3 C825T polymorphism has a significant sex-specific effect on serum soluble E-selectin levels
Authors: Kokoè Mélinda Gbadoe
J Inflamm (Lond), 2016-12-09;13(0):39.
Species: Human
Sample Types: Serum
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Independent Factors of Changes of Ankle-Brachial Index in Peripheral Arterial Occlusive Disease in Elderly Patients with or without Diabetes
Authors: Agnieszka Drosdzol-C
Int J Environ Res Public Health, 2016-11-08;13(11):.
Species: Human
Sample Types: Serum
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Vitamin D status and circulating biomarkers of endothelial dysfunction and inflammation in non-diabetic obese individuals: a pilot study
Authors: B Ilin?i?, E Stoki?, Z Sto?i?, NE Koji?, N Katsiki, DP Mikhailidi, ER Isenovic
Arch Med Sci, 2016-08-16;13(1):53-60.
Species: Human
Sample Types: Serum
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A Pilot Study to Evaluate Haemostatic Function, following Shock Wave Lithotripsy (SWL) for the Treatment of Solitary Kidney Stones.
Authors: Hughes S, Thomas-Wright S, Banwell J, Williams R, Moyes A, Mushtaq S, Abdulmajed M, Shergill I
PLoS ONE, 2015-05-04;10(5):e0125840.
Species: Human
Sample Types: Plasma
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Kidney function, endothelial activation and atherosclerosis in black and white Africans with rheumatoid arthritis.
Authors: Dessein, Patrick, Hsu, Hon-Chun, Tsang, Linda, Millen, Aletta M, Woodiwiss, Angela J, Norton, Gavin R, Solomon, Ahmed, Gonzalez-Gay, Miguel A
PLoS ONE, 2015-03-25;10(3):e0121693.
Species: Human
Sample Types: Plasma
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Immune-inflammatory markers and arterial stiffness indexes in subjects with acute ischemic stroke with and without metabolic syndrome.
Authors: Tuttolomondo A, Pecoraro R, Di Raimondo D, Di Sciacca R, Canino B, Arnao V, Butta C, Della Corte V, Maida C, Licata G, Pinto A
Diabetol Metab Syndr, 2014-02-27;6(1):28.
Species: Human
Sample Types: Plasma
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Association between traffic-related air pollution, subclinical inflammation and impaired glucose metabolism: results from the SALIA study.
Authors: Teichert T, Vossoughi M, Vierkotter A, Sugiri D, Schikowski T, Schulte T, Roden M, Luckhaus C, Herder C, Kramer U
PLoS ONE, 2013-12-10;8(12):e83042.
Species: Human
Sample Types: Plasma
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Plasma concentrations predict aortic expression of growth-arrest-specific protein 6 in patients undergoing coronary artery bypass grafting.
Authors: Lee, Chien-Hs, Shieh, Yi-Shing, Tsai, Chien-Su, Hung, Yi-Jen, Tsai, Yi-Ting, Lin, Chih-Yua
PLoS ONE, 2013-11-13;8(11):e79452.
Species: Human
Sample Types: Plasma
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Adipocytokine levels mark endothelial function in normotensive individuals.
Authors: Solini A, Stea F, Santini E, Bruno R, Duranti E, Taddei S, Ghiadoni L
Cardiovasc Diabetol, 2012-08-31;11(0):103.
Species: Human
Sample Types: Plasma
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Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis.
BMC Infect Dis, 2012-06-25;12(0):144.
Species: Human
Sample Types: Serum
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Endogenous granulocyte colony-stimulating factor: a biomarker in acute ischemic stroke.
Authors: Yu SC, Kuo CL, Huang CS, Chang CS, Wu SL, Su SL, Liu CS
Biomarkers, 2012-03-23;17(4):319-24.
Species: Human
Sample Types: Plasma
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Angiopoietin-1 and angiopoietin-2 as clinically informative prognostic biomarkers of morbidity and mortality in severe sepsis*
Authors: Ricciuto DR, Dos Santos CC, Hawkes M, Toltl LJ, Conroy AL, Rajwans N, Lafferty EI, Cook DJ, Fox-Robichaud A, Kahnamoui K, Kain KC, Liaw PC, Liles WC
Crit. Care Med., 2011-04-01;0(0):.
Species: Human
Sample Types: Plasma
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Matrix metalloproteinase-9 and plasminogen activator inhibitor-1 are associated with right ventricular structure and function: the MESA-RV Study.
Authors: Kawut SM, Barr RG, Johnson WC
Biomarkers, 2010-10-05;15(8):731-8.
Species: Human
Sample Types: Plasma
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First-trimester prediction of gestational diabetes mellitus: examining the potential of combining maternal characteristics and laboratory measures.
Authors: Savvidou M, Nelson SM, Makgoba M, Messow CM, Sattar N, Nicolaides K
Diabetes, 2010-09-28;59(12):3017-22.
Species: Human
Sample Types: Serum
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Effect of intensive lifestyle changes on endothelial function and on inflammatory markers of atherosclerosis.
Authors: Dod HS, Bhardwaj R, Sajja V, Weidner G, Hobbs GR, Konat GW, Manivannan S, Gharib W, Warden BE, Nanda NC, Beto RJ, Ornish D, Jain AC
Am. J. Cardiol., 2010-02-01;105(3):362-7.
Species: Human
Sample Types: Serum
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SIRT1 mRNA expression may be associated with energy expenditure and insulin sensitivity.
Authors: Rutanen J, Yaluri N, Modi S, Pihlajamaki J, Vanttinen M, Itkonen P, Kainulainen S, Yamamoto H, Lagouge M, Sinclair DA, Elliott P, Westphal C, Auwerx J, Laakso M
Diabetes, 2010-01-27;59(4):829-35.
Species: Human
Sample Types: Serum
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Prehospital resuscitation with hypertonic saline-dextran modulates inflammatory, coagulation and endothelial activation marker profiles in severe traumatic brain injured patients.
Authors: Rhind SG, Crnko NT, Baker AJ
J Neuroinflammation, 2010-01-18;7(0):5.
Species: Human
Sample Types: Serum
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Changes in cytokine levels during acute hyperinsulinemia in offspring of type 2 diabetic subjects.
Authors: Ruotsalainen E, Stancakova A, Vauhkonen I, Salmenniemi U, Pihlajamaki J, Punnonen K, Laakso M
Atherosclerosis, 2009-12-02;210(2):536-41.
Species: Human
Sample Types: Plasma
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Immuno-inflammatory predictors of stroke at follow-up in patients with chronic non-valvular atrial fibrillation (NVAF).
Authors: Pinto A, Tuttolomondo A, Casuccio A, Di Raimondo D, Di Sciacca R, Arnao V, Licata G
Clin. Sci., 2009-05-01;116(10):781-9.
Species: Human
Sample Types: Plasma
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Non-conventional markers of atherosclerosis before and after gastric banding surgery.
Authors: Hanusch-Enserer U, Zorn G, Wojta J, Kopp CW, Prager R, Koenig W, Schillinger M, Roden M, Huber K
Eur. Heart J., 2009-04-20;30(12):1516-24.
Species: Human
Sample Types: Plasma
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Angiopoietin-2 is associated with decreased endothelial nitric oxide and poor clinical outcome in severe falciparum malaria.
Authors: Yeo TW, Lampah DA, Gitawati R, Tjitra E, Kenangalem E, Piera K, Price RN, Duffull SB, Celermajer DS, Anstey NM
Proc. Natl. Acad. Sci. U.S.A., 2008-10-28;105(44):17097-102.
Species: Human
Sample Types: Plasma
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Immuno-inflammatory and thrombotic/fibrinolytic variables associated with acute ischemic stroke diagnosis.
Authors: Tuttolomondo A, Pinto A, Corrao S, Di Raimondo D, Fernandez P, Di Sciacca R, Arnao V, Licata G
Atherosclerosis, 2008-07-09;203(2):503-8.
Species: Human
Sample Types: Plasma
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Consumption of isoflavone-rich soy protein does not alter homocysteine or markers of inflammation in postmenopausal women.
Authors: Greany KA, Nettleton JA, Wangen KE, Thomas W, Kurzer MS
Eur J Clin Nutr, 2007-09-05;62(0):1419-25.
Species: Human
Sample Types: Serum
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Rosiglitazone produces a greater reduction in circulating platelet activity compared with gliclazide in patients with type 2 diabetes mellitus--an effect probably mediated by direct platelet PPARgamma activation.
Authors: Khanolkar MP, Morris RH, Thomas AW, Bolusani H, Roberts AW, Geen J, Jackson SK, Evans LM
Atherosclerosis, 2007-08-31;197(2):718-24.
Species: Human
Sample Types: Serum
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