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Human SCF/c-kit Ligand Antibody

Catalog #: AF-255-NA
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-255-NA
AF-255-SP
Cell Proliferation Induced by SCF/c‑kit Ligand and Neutralization by Human SCF/c‑kit Ligand Antibody.
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Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SCF/c-kit Ligand Antibody Summary

Species Reactivity
Human
Specificity
Detects human SCF/c-kit Ligand in direct ELISAs and Western blots. In direct ELISAs, less than 60% cross-reactivity with recombinant mouse SCF is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SCF/c-kit Ligand
Glu26-Ala189
Accession # P21583
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human SCF/c-kit Ligand (Catalog # 255-SC)
Immunocytochemistry
5-25 µg/mL
Immersion fixed HTC-116 Human Colorectal Carcinoma cells (Positive control) and U-937 Human Histiocytic Lymphoma cells (Negative control)
Neutralization
Measured by its ability to neutralize SCF/c‑kit Ligand-induced proliferation in the TF‑1 human erythroleukemic cell line [Kitamura, T. et al. (1989) J. Cell Physiol. 140:323]. The Neutralization Dose (ND50) is typically 0.05-0.3 µg/mL in the presence of 10 ng/mL Recombinant Human SCF/c‑kit Ligand.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by SCF/c‑kit Ligand and Neutralization by Human SCF/c‑kit Ligand Antibody. View Larger

Cell Proliferation Induced by SCF/c‑kit Ligand and Neutralization by Human SCF/c‑kit Ligand Antibody. Recombinant Human SCF/c-kit Ligand (255-SC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human SCF/c-kit Ligand (10 ng/mL) is neutralized (green line) by increasing concentrations of Human SCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA). The ND50 is typically 0.05-0.3 µg/mL.

Immunocytochemistry View Larger

Detection of SCF/c‑kit Ligand in HTC-116 cells (Positive control) and U-937 cells (Negative control). SCF/c‑kit Ligand was detected in immersion fixed HTC-116 Human Colorectal Carcinoma cells (Positive control) and absent in U-937 Human Histiocytic Lymphoma cells (Negative control) using Goat Anti-Human SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SCF/c-kit Ligand

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28-40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail. The ECD shows both N-linked and O-linked glycosylation. Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice. An alternately spliced isoform of human SCF lacks 28 aa that encompasses the primary proteolytic recognition site. Within the ECD of the short isoform (corresponding to this recombinant protein), human SCF shares 75-83% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.

Long Name
Stem Cell Factor
Entrez Gene IDs
4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)
Alternate Names
c-kit Ligand; DCUA; DFNA69; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; FPHH; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCF; SCFStem cell factor; SFc-Kit ligand; SHEP7; SLF; steel factor

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Citations for Human SCF/c-kit Ligand Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Inhibition of mast cells: a novel mechanism by which nintedanib may elicit anti-fibrotic effects
    Authors: Catherine Overed-Sayer, Elena Miranda, Rebecca Dunmore, Elena Liarte Marin, Lorea Beloki, Doris Rassl et al.
    Thorax
  2. Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
    Authors: Smith A, Bowers S, Stratman A, Davis G
    PLoS ONE, 2013-12-31;8(12):e85147.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. The fibroblast-derived paracrine factor neuregulin-1 has a novel role in regulating the constitutive color and melanocyte function in human skin.
    Authors: Choi W, Wolber R, Gerwat W
    J. Cell. Sci., 2010-08-24;123(0):3102-11.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. SCF-mediated mast cell infiltration and activation exacerbate the inflammation and immunosuppression in tumor microenvironment.
    Authors: Huang B, Lei Z, Zhang GM, Li D, Song C, Li B, Liu Y, Yuan Y, Unkeless J, Xiong H, Feng ZH
    Blood, 2008-06-04;112(4):1269-79.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Human airway epithelial cell determinants of survival and functional phenotype for primary human mast cells.
    Authors: Hsieh FH, Sharma P, Gibbons A, Goggans T, Erzurum SC, Haque SJ
    Proc. Natl. Acad. Sci. U.S.A., 2005-09-26;102(40):14380-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Growth factor displayed on the surface of retroviral particles without manipulation of envelope proteins is biologically active and can enhance transduction.
    Authors: Chandrashekran A, Gordon MY, Darling D, Farzaneh F, Casimir C
    J Gene Med, 2004-11-01;6(11):1189-96.
    Species: Human
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: ICC, Immunoprecipitation
  7. Growth differentiation factor-9 and stem cell factor promote primordial follicle formation in the hamster: modulation by follicle-stimulating hormone.
    Authors: Wang J, Roy SK
    Biol. Reprod., 2003-10-29;70(3):577-85.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15.
    Authors: Briard D, Brouty-Boye D, Azzarone B, Jasmin C
    J. Immunol., 2002-05-01;168(9):4326-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor.
    Authors: Bovolenta C, Pilotti E, Mauri M, Turci M, Ciancianaini P, Fisicaro P, Bertazzoni U, Poli G, Casoli C
    Blood, 2002-01-01;99(1):224-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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