Human S100P Antibody Summary
Met1-Lys95
Accession # P25815
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human S100P by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma parental cell line and S100P knockout HT-29 cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human S100P Monoclonal Antibody (Catalog # MAB2957) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for S100P at approximately 10 kDa (as indicated) in the parental HT-29 cell line, but is not detectable in knockout HT-29 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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S100P in NTera-2 Human Cell Line. S100P was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using 10 µg/mL Mouse Anti-Human S100P Monoclonal Antibody (Catalog # MAB2957) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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S100P Specificity is Shown by Immunocytochemistry in Knockout Cell Line. S100P was detected in immersion fixed HT-29 human colon adenocarcinoma cell line but is not detected in S100P knockout (KO) HT-29 Human Cell Line cell line using Mouse Anti-Human S100P Monoclonal Antibody (Catalog # MAB2957) at 0.1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100P
Human S100P is a 22 kDa, homodimeric member of the S100 family of calcium-binding proteins. The S100 family currently has more than 20 members and belongs to the EF-hand superfamily of molecules. S100P is 95 aa in length and contains short, sequential modules. There is an N-terminal alpha -helix, a unique EF-hand motif, an alpha -helix, a linker region, an alpha -helix, a classic EF-hand motif and a C-terminal alpha -helix. The EF-hand motif binds calcium, which likely alters molecular conformation. The rearranged S100P now binds ligand with the linker region. Human S100P shares 47% aa sequence identity with rat S100P.
Product Datasheets
Citations for Human S100P Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Down-regulation of S100P induces apoptosis in endometrial epithelial cell during GnRH antagonist protocol
Authors: Dan Zhang, Mi Han, Mingjuan Zhou, Mengyu Liu, Yan Li, Bufang Xu et al.
Reproductive Biology and Endocrinology
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An immunostaining panel of C-reactive protein, N-cadherin, and S100P is useful for intrahepatic cholangiocarcinoma subtyping
Authors: M Akita, R Sawada, M Komatsu, N Suleman, T Itoh, T Ajiki, N Heaton, T Fukumoto, Y Zen
Human pathology, 2020-12-13;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Dichotomy in intrahepatic cholangiocarcinomas based on histologic similarities to hilar cholangiocarcinomas
Authors: M Akita, K Fujikura, T Ajiki, T Fukumoto, K Otani, T Azuma, T Itoh, Y Ku, Y Zen
Mod. Pathol, 2017-03-24;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Novel quantitative analysis of the S100P protein combined with endoscopic ultrasound-guided fine needle aspiration cytology in the diagnosis of pancreatic adenocarcinoma
Authors: M Chiba, H Imazu, M Kato, K Ikeda, H Arakawa, T Kato, K Sumiyama, S Homma
Oncol. Rep, 2017-02-21;0(0):.
Species: Human
Sample Types: Tissue Homogenates
Applications: ELISA Development (Detection) -
S100P expression in response to sex steroids during the implantation window in human endometrium.
Authors: Zhang, Dan, Ma, Chengbin, Sun, Xiaoli, Xia, Hexia, Zhang, Wei
Reprod Biol Endocrinol, 2012-12-07;10(0):106.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: IHC, In-Cell Western (ICW) -
S100P-derived RAGE antagonistic peptide reduces tumor growth and metastasis.
Clin. Cancer Res., 2012-06-20;18(16):4356-64.
Species: Human
Sample Types: Cell Lysates, Recombinant Protein, Whole Tissue
Applications: ELISA Development, IHC-P, Western Blot -
Morphoproteomic profile of mTOR, Ras/Raf kinase/ERK, and NF-kappaB pathways in human gastric adenocarcinoma.
Authors: Feng W, Brown RE, Trung CD
Ann. Clin. Lab. Sci., 2008-01-01;38(3):195-209.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
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