Human S100B Antibody

Catalog #: MAB18203 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB18203-100
MAB18203-SP

Detection of S100B in Human Tonsil via Multiplex Immunofluorescence staining on COMET™

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Human S100B Antibody Summary

Species Reactivity

Human

Specificity

Detects human S100B in direct ELISAs

Source

Monoclonal Mouse IgG₁ Clone # 1046234

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human S100B synthetic peptide
Accession # P04271

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Multiplex Immunofluorescence

0.5 µg/mL

Immersion fixed paraffin-embedded sections of human tonsil

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Multiplex Immunofluorescence

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Detection of S100B in Human Tonsil via Multiplex Immunofluorescence staining on COMET™ S100B was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human S100B Monoclonal Antibody (MAB18203) at 0.5 μg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100).Specific staining was localized to the cytoplasm and nucleus. Protocol available in COMET™ Panel Builder.

Immunohistochemistry

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S100B in Human Brain (Cerebellum). S100B was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human S100B Monoclonal Antibody (Catalog # MAB18203) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in stellate cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100B

S100B belongs to the S100 subgroup of the EF-hand family of Ca²⁺ binding proteins. It is a homodimer that is expressed primarily in the brain by astrocytes, oligodendrocytes and Schwann cells. S100B has multiple intracellular functions, but can also be secreted from cells to exert extracellular functions. Some of the extracellular functions of S100B may be mediated by RAGE (receptor for advanced glycation end products). Blood levels of S100B can be used to monitor the extent of brain injury and malignant melanoma.

Long Name

S100 Calcium Binding Protein B

Entrez Gene IDs

6285 (Human)

Alternate Names

beta (neural); NEF; S100 beta; S100 calcium binding protein B; S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S-100 calcium-binding protein, beta chain, 10protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100; S100B; S100beta