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Human ROR2 Antibody

Catalog #: AF2064
15 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2064
AF2064-SP
Detection of Human ROR2 by Western Blot.
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Product Details
Citations (15)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ROR2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ROR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human ROR1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ROR2
Val28-Gly403
Accession # AAA60276
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ROR2 antibody by Western Blot. View Larger

Detection of Human ROR2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and T47D human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human ROR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2064) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ROR2 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human ROR2 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human ROR2 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ROR2 at approximately 139 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ROR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2064) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROR2

ROR2 is a type I receptor tyrosine kinase important in chondrocyte, cartilage and growth plate formation.

Long Name
Receptor Tyrosine Kinase-like Orphan Receptor 2
Entrez Gene IDs
4920 (Human); 26564 (Mouse)
Alternate Names
BDB; BDB1; EC 2.7.10.1; MGC163394; neurotrophic tyrosine kinase receptor-related 2; Neurotrophic tyrosine kinase, receptor-related 2; NTRKR2; NTRKR2tyrosine-protein kinase transmembrane receptor ROR2; receptor tyrosine kinase-like orphan receptor 2; ROR2

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Citations for Human ROR2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. The Concentration of Follistatin and Activin A in Serum and Selected Biochemical Parameters in Women with Polycystic Ovary Syndrome: Stratification by Tobacco Smoke Exposure, Insulin Resistance, and Overweight/Obesity
    Authors: Niepsuj, J;Piwowar, A;Franik, G;Bizo?, A;
    Journal of clinical medicine
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Two functional forms of the Meckel-Gruber syndrome protein TMEM67 generated by proteolytic cleavage by ADAMTS9 mediate Wnt signaling and ciliogenesis
    Authors: Ahmed, M;Fischer, S;Robert, KL;Lange, KI;Stuck, MW;Best, S;Johnson, CA;Pazour, GJ;Blacque, OE;Nandadasa, S;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. ROR2 expression predicts human induced pluripotent stem cell differentiation into neural stem/progenitor cells and GABAergic neurons
    Authors: Kuroda, T;Yasuda, S;Matsuyama, S;Miura, T;Sawada, R;Matsuyama, A;Yamamoto, Y;Morioka, MS;Kawaji, H;Kasukawa, T;Itoh, M;Akutsu, H;Kawai, J;Sato, Y;
    Scientific reports
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Cancer-associated fibroblasts influence Wnt/PCP signaling in gastric cancer cells by cytoneme-based dissemination of ROR2
    Authors: Rogers, S;Zhang, C;Anagnostidis, V;Liddle, C;Fishel, ML;Gielen, F;Scholpp, S;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Interpreting ciliopathy-associated missense variants of uncertain significance (VUS) in Caenorhabditis elegans
    Authors: Karen I Lange, Sunayna Best, Sofia Tsiropoulou, Ian Berry, Colin A Johnson, Oliver E Blacque
    Human Molecular Genetics
  6. The Cell Surface Receptors Ror1/2 Control Cardiac Myofibroblast Differentiation
    Authors: Nicholas W. Chavkin, Soichi Sano, Ying Wang, Kosei Oshima, Hayato Ogawa, Keita Horitani et al.
    Journal of the American Heart Association
  7. Receptor tyrosine kinase-like orphan receptor 2 (Ror2) expression creates a poised state of Wnt signaling in renal cancer.
    Authors: Rasmussen N, Wright T, Brooks S, Hacker K, Debebe Z, Sendor A, Walker M, Major M, Green J, Wahl G, Rathmell W
    J Biol Chem, 2013-07-26;288(36):26301-10.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Targeting malignant B cells with an immunotoxin against ROR1
    Authors: Sivasubramanian Baskar, Adrian Wiestner, Wyndham H. Wilson, Ira Pastan, Christoph Rader
    mAbs
  9. Ror family receptor tyrosine kinases regulate the maintenance of neural progenitor cells in the developing neocortex.
    J. Cell. Sci., 2012-02-10;125(0):2017-29.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Therapeutic Potential and Challenges of Targeting Receptor Tyrosine Kinase ROR1 with Monoclonal Antibodies in B-Cell Malignancies.
    Authors: Yang J, Baskar S, Kwong KY
    PLoS ONE, 2011-06-15;6(6):e21018.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development
  11. Wnt5a/Ror2-induced upregulation of xPAPC requires xShcA.
    Authors: Feike AC, Rachor K, Gentzel M, Schambony A
    Biochem. Biophys. Res. Commun., 2010-08-21;400(4):500-6.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  12. The Wnt-5a-derived hexapeptide Foxy-5 inhibits breast cancer metastasis in vivo by targeting cell motility.
    Authors: Safholm A, Tuomela J, Rosenkvist J, Dejmek J, Harkonen P, Andersson T
    Clin. Cancer Res., 2008-10-15;14(20):6556-63.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Unique cell surface expression of receptor tyrosine kinase ROR1 in human B-cell chronic lymphocytic leukemia.
    Authors: Baskar S, Kwong KY, Hofer T, Levy JM, Kennedy MG, Lee E, Staudt LM, Wilson WH, Wiestner A, Rader C
    Clin. Cancer Res., 2008-01-15;14(2):396-404.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. A regulatory circuit of miR-125b/miR-20b and Wnt signalling controls glioblastoma phenotypes through FZD6-modulated pathways
    Authors: Tianzhi Huang, Angel A. Alvarez, Rajendra P. Pangeni, Craig M M. Horbinski, Songjian Lu, Sung-Hak Kim et al.
    Nature Communications
  15. Regulation of lipid synthesis by the RNA helicase Mov10 controls Wnt5a production.
    Authors: W Wang, N Snyder, A J Worth et al.
    Oncogenesis

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