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Human Renin Antibody

Catalog #: AF4090
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4090
AF4090-SP
Renin in Human Kidney.
2 Images
Product Details
Citations (3)
FAQs
Supplemental Products
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Renin Antibody Summary

Species Reactivity
Human
Specificity
Detects both pro and mature forms of human Renin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse Renin-1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Renin
Leu24-Arg406
Accession # P60016
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Renin (Catalog # 4090-AS)
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Renin (Catalog # 4090‑AS), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Human Renin (10 µg/mL, Catalog # 4090-AS) cleavage of the fluorogenic peptide substrate Renin Substrate I (10 µM). The Neutralization Dose (ND50) is typically 100 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Renin antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

Renin in Human Kidney. Renin was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human Renin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4090) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Renin antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

Renin in Human Kidney. Renin was detected in immersion fixed paraffin-embedded sections of human kidney array using Sheep Anti-Human Renin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4090) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Renin

Human Renin is a member of the aspartyl proteinase family produced largely in part by the juxtaglomerular cells in the kidney (1). Renin differs from the other members of this class by having a pH optimum near the neutral pH region with native substrates instead of a pH 2.0 to 3.4 range (2). This more neutral pH optimum allows it to be functional in the plasma. Renin also has a very high selectivity for substrates due to a long peptide recognition on either side of the peptide bond undergoing cleavage. An octapeptide substrate was the minimum length to be cleaved by Renin. Renin plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen, which is the only known physiological substrate of Renin. Renin releases the decapeptide angiotensin I, which in turn is further converted to vasoactive hormone angiotensin II by angiotensin converting enzyme (ACE). Renin is produced as prorenin with 43 pro residues at the N‑terminal of mature Renin. The inactive prorenin becomes activated proteolytically by trypsin, cathepsin B, or other proteinases.

References
  1. Yokosawa, H. et al. (1980) J. Biol. Chem. 255:3498.
  2. Fuminaki, S. et al. (2004) in Handbook of Proteolytic Enzymes, Barret, A. J. et al. eds. p. 54.
Entrez Gene IDs
5972 (Human); 19701 (Mouse)
Alternate Names
angiotensin-forming enzyme; Angiotensinogenase; EC 3.4.23; EC 3.4.23.15; FLJ10761; HNFJ2; Prorenin; REN; renin precursor, renal; Renin

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Citations for Human Renin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. A single-cell survey of the human first-trimester placenta and decidua
    Authors: H Suryawansh, P Morozov, A Straus, N Sahasrabud, KEA Max, A Garzia, M Kustagi, T Tuschl, Z Williams
    Sci Adv, 2018-10-31;4(10):eaau4788.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Human kidney pericytes produce renin
    Kidney Int, 2016-09-24;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. Expression of classical components of the renin-angiotensin system in the human eye
    Authors: Andrew J R White, Sarat C Cheruvu, Maria Sarris, Surabhi S Liyanage, Eugenie Lumbers, Jeanie Chui et al.
    Journal of the Renin-Angiotensin-Aldosterone System

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