Summary
Precision
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Human Relaxin-2 Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
4.57 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Relaxin-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Relaxin-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Relaxin-2 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Relaxin-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Relaxin-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Relaxin-2.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 41 | 41 | 41 |
| Mean (pg/mL) | 35.1 | 96 | 194 | 34.9 | 98.9 | 197 |
| Standard Deviation | 1.27 | 2.44 | 4.01 | 3.41 | 5.9 | 11.7 |
| CV% | 3.6 | 2.5 | 2.1 | 9.8 | 6 | 5.9 |
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 42 | 42 | 42 |
| Mean (pg/mL) | 34.4 | 99.6 | 206 | 40.8 | 112 | 220 |
| Standard Deviation | 1.6 | 2.47 | 4.75 | 4.16 | 7.02 | 12.1 |
| CV% | 4.7 | 2.5 | 2.3 | 10.2 | 6.3 | 5.5 |
Recovery
The recovery of Relaxin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 89-102 |
| EDTA Plasma (n=4) | 98 | 93-106 |
| Heparin Plasma (n=4) | 97 | 91-104 |
| Serum (n=4) | 101 | 92-107 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Relaxin-2 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Relaxin-2
The Insulin/Relaxin superfamily includes Insulin, IGF-I, IGF-II, Relaxins, Relaxin-like Factor (RLF), Early Placenta Insulin-like Peptide 4 (INSL4), INSL5, and INSL6. Three human Relaxin genes (Relaxins-1 to -3) have been identified. Relaxin-2 is secreted and circulates in the blood, whereas Relaxin-1 is expressed in decidua, placenta, and prostate, and Relaxin-3 is expressed in the brain. Human Relaxin-2 is a peptide hormone that plays an important role in female reproduction.
Entrez Gene IDs:
6019 (Human)
Alternate Names:
bA12D24.1.1; bA12D24.1.2; H2; relaxin 2; Relaxin2; Relaxin-2; RLN2; RLXH2
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
50 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Citations for Human Relaxin-2 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus
Authors: MVC Rudge, FP Souza, JF Abbade, RLS Hallur, JPC Marcondes, F Piculo, G Marini, G Vesentini, L Thabane, SS Witkin, IMP Calderon, AMP Barbosa, MV Rudge, AMP Barbosa, IMP Calderon, FP Souza, JF Abbade, LSR Hallur, F Piculo, G Marini, G Vesentini, L Thabane, MS Palma, CFO Graeff, RK Arni, RD Herculano, DF Salvadori, S Mateus, M Dal Pai Si, CG Magalhães, RA Costa, SAM Lima, SL Felisbino, W Barbosa, A Atallah, MJB Girão, Z Di Bella, SM Uchoa, S Payão, A Hijas, B Berghman, R De Bie, L Sobrevia, B Junginger, FCB Alves, PS Rossignoli, CB Prudencio, MIG Orlandi, MI Gonçalves, SK Nunes, BB Catinelli, S Quiroz, BV Sarmento, FA Pinheiro, CI Sartorão, RR Lucas, DRA Reyes, SBCV Quiroz, EMA Enriquez, RG Oliveira, JF Floriano, JPC Marcondes, S Barneze, TD Dangió, T Pascon, P Rossignoli, JV Freitas, L Takano, F Reis, TD Caldeirão, JN Fernandes, AM Carr, MVC Gaitero, JE Corrente, HRC Nunes, AF Candido, SMB Costa, TD Dangió, T Pascon, JVF Melo, L Takano, FVDS Reis, TD Caldeirão, AM Carr, GA Garcia, GB Rabadan, HCM Bassin, KS Suyama, LN Damasceno, MLS Takemoto, MD Menezes, DG Bussaneli, VKC Nogueira, PR Lima, IO Lourenço, J Marostica, RA Megid, IP Caruso, LT Rasmussen, GM Prata, F Piculo, G Vesentini, MA Arantes, GAR Ferraz, LP Camargo, MR Kron, JE Corrente, HRC Nunes
BMC Pregnancy Childbirth, 2020-02-19;20(1):117.
Species: Human
Sample Types: Serum
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Serelaxin Improves Regional Myocardial Function in Experimental Heart Failure: An In Vivo Cardiac Magnetic Resonance Study
Authors: T Lapinskas, S Kelle, J Grune, A Foryst-Lud, H Meyborg, S Jeuthe, E Wellnhofer, A Elsanhoury, B Pieske, R Gebker, U Kintscher, P Stawowy
J Am Heart Assoc, 2020-01-31;9(3):e013702.
Species: Mouse
Sample Types: Plasma
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Relaxin reverses maladaptive remodeling of the aged heart through Wnt-signaling
Authors: B Martin, B Gabris, AF Barakat, BL Henry, M Giannini, RP Reddy, X Wang, G Romero, G Salama
Sci Rep, 2019-12-06;9(1):18545.
Species: Rat
Sample Types: Plasma
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Circulating Relaxin-1 Level Is a Surrogate Marker of Myocardial Fibrosis in HFrEF
Authors: J Simon, E Nemeth, A Nemes, M Husveth-To, T Radovits, G Foldes, L Kiss, Z Bagyura, J Skopal, B Merkely, E Gara
Front Physiol, 2019-06-04;10(0):690.
Species: Human
Sample Types: Serum
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Cardiac magnetic resonance using late gadolinium enhancement and atrial T1 mapping predicts poor outcome in patients with atrial fibrillation after catheter ablation therapy
Authors: JA Luetkens, AC Wolpers, T Beiert, D Kuetting, D Dabir, R Homsi, H Meenderman, NA Dayé, V Knappe, M Karsdal, SH Nielsen, F Genovese, F Stöckigt, M Linhart, D Thomas, G Nickenig, HH Schild, JW Schrickel, RP Andrié
Sci Rep, 2018-09-11;8(1):13618.
Species: Human
Sample Types: Plasma
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Associations between serum relaxin 2, aneurysm formation/size and severity of atherosclerosis: a preliminary prospective analysis
Authors: K Papoutsis, A Kapelouzou, DI Tsilimigra, N Patelis, G Kouvelos, D Schizas, I Karavokyro, S Georgopoul
Acta Pharmacol. Sin., 2018-03-22;0(0):.
Species: Human
Sample Types: Serum
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Short-term (48 hours) intravenous serelaxin infusion has no effect on myogenic tone or vascular remodeling in rat mesenteric arteries
Authors: M Jelinic, CH Leo, SA Marshall, SN Senadheera, LJ Parry, M Tare
Microcirculation, 2017-08-01;0(0):.
Species: Rat
Sample Types: Plasma
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Reperfusion therapy with recombinant human relaxin-2 (Serelaxin) attenuates myocardial infarct size and NLRP3 inflammasome following ischemia/reperfusion injury via eNOS-dependent mechanism
Authors: J Valle Rale, AG Mauro, T Devarakond, C Marchetti, J He, E Kim, S Filippone, A Das, S Toldo, A Abbate, FN Salloum
Cardiovasc. Res, 2017-05-01;0(0):.
Species: Human
Sample Types: Plasma
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Serelaxin as a potential treatment for renal dysfunction in cirrhosis: Preclinical evaluation and results of a randomized phase 2 trial
Authors: VK Snowdon, NJ Lachlan, AM Hoy, PW Hadoke, SI Semple, D Patel, W Mungall, TJ Kendall, A Thomson, RJ Lennen, MA Jansen, CM Moran, A Pellicoro, P Ramachandr, I Shaw, RL Aucott, T Severin, R Saini, J Pak, D Yates, N Dongre, JS Duffield, DJ Webb, JP Iredale, PC Hayes, JA Fallowfiel
PLoS Med, 2017-02-28;14(2):e1002248.
Species: Human, Rat
Sample Types: Serum
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Serelaxin treatment reverses vascular dysfunction and left ventricular hypertrophy in a mouse model of Type 1 diabetes
Authors: HH Ng, CH Leo, D Prakoso, C Qin, RH Ritchie, LJ Parry
Sci Rep, 2017-01-09;7(0):39604.
Species: Mouse
Sample Types: Plasma
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Localization of relaxin receptors in arteries and veins, and region-specific increases in compliance and bradykinin-mediated relaxation after in vivo serelaxin treatment.
Authors: Jelinic M, Leo C, Post Uiterweer E, Sandow S, Gooi J, Wlodek M, Conrad K, Parkington H, Tare M, Parry L
FASEB J, 2013-09-13;28(1):275-87.
Species: Rat
Sample Types: Plasma
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Relaxin regulates hyaluronan synthesis and aquaporins in the cervix of late pregnant mice.
Authors: Soh, Yu May, Tiwari, Anjana, Mahendroo, Mala, Conrad, Kirk P, Parry, Laura J
Endocrinology, 2012-10-19;153(12):6054-64.
Species: Human
Sample Types: Complex Sample Type
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Serum relaxin levels are reduced in pregnant women with a history of recurrent miscarriage, and correlate with maternal uterine artery Doppler indices in first trimester.
Authors: Anumba DO, El Gelany S, Elliott SL
Eur. J. Obstet. Gynecol. Reprod. Biol., 2009-08-19;147(1):41-5.
Species: Human
Sample Types: Serum
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