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Human RelA/NFkB p65 Antibody

Catalog #: MAB50781
1 Citation Datasheet / COA / SDS
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MAB50781
MAB50781-SP
Detection of RelA/NF kappa B p65 in Daudi Human Cell Line by Flow Cytometry.
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Human RelA/NFkB p65 Antibody Summary

Species Reactivity
Human
Specificity
Detects human RelA/NF kappa B p65 in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 465003
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human RelA/NF kappa B p65
Met1-Thr254
Accession # NP_068810
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of RelA/NF?B p65 antibody in Daudi Human Cell Line antibody by Flow Cytometry. View Larger

Detection of RelA/NF kappa B p65 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Human RelA/NF kappa B p65 Monoclonal Antibody (Catalog # MAB50781, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RelA/NFkB p65

RelA p65 is a 65 kDa member of the NFkB family of nuclear transcription factors. Dimers of p65 with the p50 subunit are the most common form of the NFkB transcription factor, but dimers with itself or other family members are also active. An alternatively spliced isoform that lacks amino acids (aa) 222-231 (p65D) does not bind DNA. Over the sequence used as an immunogen, human RelA p65 shares 96% and 98% aa identity with mouse and rat RelA p65, respectively. This portion includes one of eight potential Ser/Thr phosphorylation sites, two acetylation sites, and most of the Rel homology domain that interacts with IkB inhibitors.

Long Name
v-rel Reticuloendotheliosis Viral Oncogene Homolog A
Entrez Gene IDs
5970 (Human)
Alternate Names
MGC131774; nf kb p65; NFkB p65; NF-kB p65; NFKB3; NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65)); Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65; p65; p65RelA; rela p65; RelA; v-rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

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Citation for Human RelA/NFkB p65 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Buyang Huanwu Tang improves denervation-dependent muscle atrophy by increasing ANGPTL4, and increases NF-?B and MURF1 levels
    Authors: L Zhou, Y Huang, H Xie, X Mei
    Mol Med Rep, 2017-12-19;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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