Human/Rat/Primate Phospho-5-Lipoxygenase (S523) Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: 5-Lipoxygenase

5-Lipoxygenase (5-LO) is an 80 kDa, 676 amino acid (aa) nonheme iron dioxygenase. It converts nuclear membrane arachidonic acid into leukotriene A4 (LTA₄) that is then modified to produce either LTB₄ or LTC₄. 5-LO is found in leukocytes and generally exists in the cytoplasm. Upon activation, 5-LO will either remain cytosolic or undergo translocation into the nucleus. In the nucleus, its enzyme products impact gene expression; in the cytosol, its products are secreted and contribute to inflammation. 5-LO has three Nuclear Localization Signal (NLS) motifs starting at amino acids 112, 158, and 518. Upon cell activation, Ser at 523 is phosphorylated by Protein Kinase A (PKA). This amino acid lies within the principal NLS of 5-LO. When phosphorylated, the NLS is inactivated, blocking 5-LO entry into the nucleus. This appears to impact overall leukotriene biosynthesis, and potentially serves as a regulatory mechanism for inflammation.

Product Datasheets

Related Research Areas

• Additional Reduction/Oxidation (Redox) Enzymes
• Bioactive Lipids and Receptors
• Eicosanoids and Regulators
• Lipid/Cholesterol Metabolism Enzymes
• Neurotransmitters, Neuroactive Molecules and Associated Enzymes
• Oxidases and Oxygenases
• Oxygenases/Hydroxylases
• Pro-oxidant Molecules
• Redox Enzymes
• Steroid Hormones and Bioactive Lipids