Human/Rat CCR4 APC-conjugated Antibody
Human/Rat CCR4 APC-conjugated Antibody Summary
Met1-Leu360
Accession # P51679
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of CCR4 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. Detection of CCR4 antibody in Human Blood Lymphocytes antibody by Flow Cytometry.](https://resources.rndsystems.com/images/datasheets/antibody/CCR4_FAB1567A_Flow_Cytometry_16965.jpg)
Detection of CCR4 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human/Rat CCR4 APC-conjugated Monoclonal Antibody (Catalog # FAB1567A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
![Detection of CCR4 antibody in Rat Splenocytes antibody by Flow Cytometry. Detection of CCR4 antibody in Rat Splenocytes antibody by Flow Cytometry.](https://resources.rndsystems.com/images/datasheets/antibody/CCR4_FAB1567A_Flow_Cytometry_14909.jpg)
Detection of CCR4 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Mouse Anti-Human/Rat CCR4 APC-conjugated Monoclonal Antibody (Catalog # FAB1567A) and Anti-Rat CD3e PE-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR4
CCR4 is a G protein-linked seven transmembrane domain chemokine receptor that binds the chemokines CCL17/TARC and CCL22/MDC. Current evidence suggests that CCR4 expression is associated with Th-2 type T cells and with platelets. CCR4 expression has also been reported in mature dendritic cells.
Product Datasheets
Citations for Human/Rat CCR4 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Activated CLL cells regulate IL17F producing Th17 cells in miR155 dependent and outcome specific manners
Authors: B Jung, G Ferrer, PY Chiu, R Aslam, A Ng, F Palacios, M Wysota, M Cardillo, JE Kolitz, SL Allen, JC Barrientos, KR Rai, N Chiorazzi, B Sherry
JCI Insight, 2022-06-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
CCR2 identifies a stable population of human effector memory CD4+ T cells equipped for rapid recall response.
Authors: Zhang HH, Song K, Rabin RL, Hill BJ, Perfetto SP, Roederer M, Douek DC, Siegel RM, Farber JM
J. Immunol., 2010-10-27;185(11):6646-63.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Elevated frequencies of highly activated CD4+ T cells in HIV+ patients developing immune reconstitution inflammatory syndrome.
Authors: Antonelli LR, Mahnke Y, Hodge JN, Porter BO, Barber DL, DerSimonian R, Greenwald JH, Roby G, Mican J, Sher A, Roederer M, Sereti I
Blood, 2010-07-26;116(19):3818-27.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
Authors: Ahern D, Lloyd CM, Robinson DS
Allergy, 2009-02-07;64(8):1121-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion.
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100