Human PSENEN Antibody

Catalog #: MAB6859 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6859
MAB6859-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PSENEN Antibody Summary

Species Reactivity

Human

Specificity

Detects human PSENEN in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 665521

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Peptide containing human PSENEN
Gly89-Gly98
Accession # Q9NZ42

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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PSENEN in Human Brain. PSENEN was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Human PSENEN Monoclonal Antibody (Catalog # MAB6859) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and neurites of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PSENEN

Gamma-secretase subunit PSENEN, also called PEN-2 (Presenilin enhancer protein 2) is a 10 kDa member of the PEN-2 family. Human PSENEN is 101 amino acids (aa) in length, and is a multi-pass transmembrane protein. Residues 1-17 are on the luminal side of the endoplasmic reticulum or Golgi apparatus, where PSENEN is primarily located. Amino acids 18-38 form a transmembrane region, and aa 39-60 form a cytoplasmic segment. Another transmembrane segment is formed by residues 61-81, and residues 82-101 are located on the luminal side of the ER or cis-Golgi. Human PSENEN shares 96% aa sequence identity with mouse and rat PSENEN. Functionally, PSENEN is an essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and beta-amyloid precursor protein.

Long Name

Presenilin Enhancer 2 Homolog

Entrez Gene IDs

55851 (Human); 66340 (Mouse); 292788 (Rat)

Alternate Names

gamma-secretase subunit PEN-2; MSTP064; PEN-2; PEN2hematopoietic stem/progenitor cells protein MDS033; presenilin enhancer 2 homolog (C. elegans); Presenilin enhancer protein 2; PSENEN