Human Pro-Corticoliberin/Pro-CRH Antibody

Catalog #: AF5805 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5805
AF5805-SP

Detection of Human Pro-Corticoliberin/Pro-CRH by Western Blot.

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All Pro-Corticoliberin/Pro-CRH Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Pro-Corticoliberin/Pro-CRH Antibody Summary

Species Reactivity

Human

Specificity

Detects human Pro‑Corticoliberin/Pro‑CRH in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Pro‑Corticoliberin/Pro‑CRH
Leu25-Lys196
Accession # P06850

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Pro-Corticoliberin/Pro-CRH by Western Blot. Western blot shows lysates of human cerebellum and hypothalamus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pro-Corticoliberin/Pro-CRH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5805) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pro-Corticoliberin/Pro-CRH at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pro-Corticoliberin/Pro-CRH

Pro-Corticoliberin (also Pro-CRF and Pro-CRH) is a predicted 19 kDa member of the CRF/Urotensin I family of hormones. It is expressed by placental synctiotrophoblasts, zona fasciculata adrenal cells and paraventricular neurons, and is induced by glucocorticoids. Pro‑Corticoliberin is the precursor to a mature, 41 amino acid (aa) amidated Corticoliberin that induces pituitary ACTH and subsequent cortisol production by the adrenal. Human Pro‑Corticoliberin is 170 aa in length (aa 25‑194). If unprocessed, it undergoes N-terminal amidation to generate a polypeptide 168 aa in length (aa 27‑194). Secreted Pro‑Corticoliberin is both bioactive and binds to 37 kDa Corticoliberin-PB. In the placenta, Pro‑Corticoliberin is only occasionally cleaved to generate mature Corticoliberin (aa 152‑192); in the brain, it undergoes active internal proteolysis to generate multiple fragments, including the 5 kDa mature peptide. Over aa 25‑196, human Pro‑Corticoliberin shares 78% aa identity with mouse Pro‑Corticoliberin.

Long Name

Pro-Corticotropin-releasing Hormone

Entrez Gene IDs

1392 (Human); 12918 (Mouse); 81648 (Rat)

Alternate Names

CRF; CRH; CRH1; ProCorticoliberin; Pro-Corticoliberin