Human Pro-Cathepsin B Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Cell Lysates, Saliva, Urine
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 1.08 | 2.39 | 4.5 | 1.16 | 2.58 | 4.91 |
Standard Deviation | 0.05 | 0.11 | 0.23 | 0.1 | 0.16 | 0.3 |
CV% | 4.6 | 4.6 | 5.1 | 8.6 | 6.2 | 6.1 |
Serum, Heparin Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 1.32 | 2.93 | 5.78 | 1.48 | 3.18 | 5.95 |
Standard Deviation | 0.08 | 0.15 | 0.29 | 0.13 | 0.24 | 0.42 |
CV% | 6.1 | 5.1 | 5 | 8.8 | 7.5 | 7.1 |
Recovery
The recovery of Pro-Cathepsin B spiked to levels throughout the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 106 | 98-115 |
Linearity
Scientific Data
![Human Pro-Cathepsin B ELISA Cell Culture Supernate/Cell Lysate/Saliva/Urine Standard Curve Human Pro-Cathepsin B ELISA Cell Culture Supernate/Cell Lysate/Saliva/Urine Standard Curve](https://resources.rndsystems.com/images/datasheets/elisa/DATA_Cathepsin_B_DCATB0_ELISA_609.jpg)
Human Pro-Cathepsin B ELISA Cell Culture Supernate/Cell Lysate/Saliva/Urine Standard Curve
Product Datasheets
Preparation and Storage
Background: Cathepsin B
Cathepsin B is the first described member of the family of lysosomal cysteine proteases. Cathepsin B possesses both endopeptidase and exopeptidase activities, in the latter case acting as a peptidyl-dipeptidase. It is known to process a number of proteins, including pro and active caspases, prorenin and SLPI. Cathepsin B is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at 2-8 °C for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
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Citations for Human Pro-Cathepsin B Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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Dysregulation of macrophage-secreted cathepsin B contributes to HIV-1-linked neuronal apoptosis.
Authors: Rodriguez-Franco EJ, Cantres-Rosario YM, Plaud-Valentin M, Romeu R, Rodriguez Y, Skolasky R, Melendez V, Cadilla CL, Melendez LM
PLoS ONE, 2012-05-31;7(5):e36571.
Species: Human
Sample Types: Cell Culture Supernates
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A possible contribution of altered cathepsin B expression to the development of skin sclerosis and vasculopathy in systemic sclerosis.
Authors: Noda S, Asano Y, Akamata K, Aozasa N, Taniguchi T, Takahashi T, Ichimura Y, Toyama T, Sumida H, Yanaba K, Tada Y, Sugaya M, Kadono T, Sato S
PLoS ONE, 2012-02-23;7(2):e32272.
Species: Human
Sample Types: Serum
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Identification of candidate nasopharyngeal carcinoma serum biomarkers by cancer cell secretome and tissue transcriptome analysis: potential usage of cystatin A for predicting nodal stage and poor prognosis.
Authors: Chang KP, Wu CC, Chen HC
Proteomics, 2010-07-01;10(14):2644-60.
Species: Human
Sample Types: Serum
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Decreased lubricin concentrations and markers of joint inflammation in the synovial fluid of patients with anterior cruciate ligament injury.
Authors: Elsaid KA, Fleming BC, Oksendahl HL, Machan JT, Fadale PD, Hulstyn MJ, Shalvoy R, Jay GD
Arthritis Rheum., 2008-06-01;58(6):1707-15.
Species: Human
Sample Types: Synovial Fluid
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Imbalanced serum concentration between cathepsin B and cystatin C in patients with pelvic inflammatory disease.
Authors: Tsai HT, Wang PH, Tee YT, Lin LY, Hsieh YS, Yang SF
Fertil. Steril., 2008-03-07;91(2):549-55.
Species: Human
Sample Types: Serum
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Mesenchymal stem cells support migration, extracellular matrix invasion, proliferation, and survival of endothelial cells in vitro.
Authors: Potapova IA, Gaudette GR, Brink PR, Robinson RB, Rosen MR, Cohen IS, Doronin SV
Stem Cells, 2007-03-29;25(7):1761-8.
Species: Human
Sample Types: Cell Culture Supernates
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Generation of endostatin by matrix metalloproteinase and cathepsin from human limbocorneal epithelial cells cultivated on amniotic membrane.
Authors: Ma DH, Yao JY, Kuo MT, See LC, Lin KY, Chen SC, Chen JK, Chao AS, Wang SF, Lin KK
Invest. Ophthalmol. Vis. Sci., 2007-02-01;48(2):644-51.
Species: Human
Sample Types: Cell Culture Supernates
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Acidic extracellular pH promotes experimental metastasis of human melanoma cells in athymic nude mice.
Authors: Rofstad EK, Mathiesen B, Kindem K, Galappathi K
Cancer Res., 2006-07-01;66(13):6699-707.
Species: Human
Sample Types: Cell Culture Supernates
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Serum level of cathepsin B and its density in men with prostate cancer as novel markers of disease progression.
Authors: Miyake H, Hara I, Eto H
Anticancer Res., 2004-07-01;24(4):2573-7.
Species: Human
Sample Types: Serum
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