Human Pro‑BNP Antibody

Catalog #: MAB36041 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB36041
MAB36041-SP

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Human Pro‑BNP Antibody Summary

Species Reactivity

Human

Specificity

Detects human Pro‑BNP in direct ELISAs. Does not detect BNP-32 (aa 103‑134).

Source

Monoclonal Rat IgG2b Clone # 370124

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Human Pro-BNP

Immunohistochemistry

8-25 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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BNP in BG01V Human Cells. BNP was detected in immersion fixed BG01V human embryonic stem cells using Rat Anti-Human Pro-BNP Monoclonal Antibody (Catalog # MAB36041) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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Pro-BNP in Human Heart. Pro-BNP was detected in immersion fixed paraffin-embedded sections of human heart using 25 µg/mL Rat Anti-Human Pro-BNP Monoclonal Antibody (Catalog # MAB36041) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to endothelial cells in the vasculature. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: BNP

Brain-type Natriuretic Peptide (BNP) is a nonglycosylated peptide that is produced predominantly by ventricular myocytes and belongs to the natriuretic peptide family. Proteolytic cleavage of the 12 kDa BNP precursor gives rise to N-terminal Pro‑BNP (NT-proBNP) and mature BNP. Plasma NT-proBNP is a marker for congestive heart failure, while mature BNP (aa 103‑134) promotes vasodilation and fluid and sodium excretion. Human BNP precursor shares 29% and 51% aa sequence identity with mouse and porcine BNP precursor, respectively.

Long Name

Brain Natriuretic Peptide

Entrez Gene IDs

4879 (Human); 18158 (Mouse); 25105 (Rat)

Alternate Names

BNF; BNP; brain type natriuretic peptide; Gamma-brain natriuretic peptide; natriuretic peptide B; natriuretic peptide precursor B; natriuretic peptides B; natriuretic protein; NPPB