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Human Pref-1/DLK1/FA1 DuoSet ELISA

Catalog #: DY1144-05
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY1144-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human Pref-1 / DLK1 / FA1 ELISA Standard Curve
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Product Details
Citations (1)
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human Pref-1/DLK1/FA1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
93.8 - 6,000 pg/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Pref-1 / DLK1 / FA1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A Human Pref-1 / DLK1 / FA1 ELISA Standard Curve View Larger

Human Pref-1 / DLK1 / FA1 ELISA Standard Curve

Product Datasheets

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Product Datasheet
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SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Pref-1/DLK1/FA1

Pref-1 (preadipocyte factor 1), also known as deltalike 1 (DLK1) and fetal antigen 1 (FA1), is a member of the Notch/Delta/Serrate protein family. It is a transmembrane protein that can be proteolyically cleaved to generate soluble forms. Pref-1 is believed to be a co-ligand for Notch signaling, enhancing or inhibiting Notch activation following binding of a ligand. It is expressed in numerous endocrine cells, and is highly expressed in preadipocytes, but not in mature adipocytes. In adipose tissue, Pref-1 inhibits adipogenesis and is often used as a preadipocyte marker.

Long Name:
Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1
Entrez Gene IDs:
8788 (Human); 13386 (Mouse)
Alternate Names:
delta-like 1 homolog (Drosophila); DLK; DLK1; DLK-1; FA1; FA1fetal antigen 1; pG2; pG2delta-like homolog (Drosophila); preadipocyte factor 1; Pref1; Pref-1; protein delta homolog 1; secredeltin; ZOG

Citation for Human Pref-1/DLK1/FA1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Serum levels of the adipokine Pref-1 in gestational diabetes mellitus.
    Authors: Wurst U, Ebert T, Kralisch S, Stumvoll M, Fasshauer M
    Cytokine, 2014-11-17;71(2):161-4.
    Species: Human
    Sample Types: Serum

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