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Human PP2C gamma Antibody

Catalog #: MAB5595 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5595
MAB5595-SP
Detection of Human PP2C gamma /PPM1G by Western Blot.
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Human PP2C gamma Antibody Summary

Species Reactivity
Human
Specificity
Detects human PP2C gamma.
Source
Monoclonal Mouse IgG1 Clone # 528322
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PP2C gamma
Met1-Asp546
Accession # O15355
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human liver and human liver cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PP2C?/PPM1G antibody by Western Blot. View Larger

Detection of Human PP2C gamma /PPM1G by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Human PP2C gamma/PPM1G Monoclonal Antibody (Catalog # MAB5595) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PP2C gamma/PPM1G at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PP2C gamma

Protein phosphatase 2C isoform gamma (PP2C gamma; also PPM1G) is a widely expressed, 75 kDa member of the PP2C family of Ser/Thr protein phosphatases. Human PP2C gamma is 546 amino acids (aa) in length. The apparent mass is much larger than the predicted mass of 59 kDa because of the presence of an acidic region (aa 259‑326) rich in glutamate and aspartate residues, causing the protein to move anomalously on SDS-PAGE. The protein contains numerous sites for phosphorylation by casein kinase II, particularly within the acidic domain.

Long Name
Protein Phosphatase 2C gamma
Entrez Gene IDs
5496 (Human); 14208 (Mouse); 259229 (Rat)
Alternate Names
EC 3.1.3.16; MGC1675; MGC2870; PP2C gamma; PP2C, gamma; PP2CG; PP2CGAMMA; PP2C-gamma; PPM1C; PPM1G; PPP2CG; Protein phosphatase 1C; protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform; protein phosphatase 1G; protein phosphatase 2, catalytic subunit, gamma isoform; protein phosphatase 2C gamma isoform; Protein phosphatase 2C isoform gamma; Protein phosphatase magnesium-dependent 1 gamma; protein phosphatase, Mg2+/Mn2+ dependent, 1G

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Human PP2C gamma Antibody
By Anonymous on 05/20/2022
Application: IHC Sample Tested: Pancreas tissue Species: Human
Immunohistochemistry Human PP2C gamma Antibody MAB5595