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Human PLZF APC-conjugated Antibody

Catalog #: IC2944A
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC2944A
Detection of PLZF in HL‑60 Human Cell Line by Flow Cytometry.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PLZF APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human PLZF in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ZBTB38, rhZNF24, rhZNF143, rhZNF206, rhZNF281, or rhZNF423 is observed.
Source
Monoclonal Mouse IgG2A Clone # 6318100
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PLZF
Met1-Gln254
Accession # Q05516
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of PLZF antibody in HL-60 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of PLZF in HL‑60 Human Cell Line by Flow Cytometry. HL-60 human acute promyelocytic leukemia cell line was stained with Mouse Anti-Human PLZF APC-conjugated Monoclonal Antibody (Catalog # IC2944A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: PLZF

Human PLZF is a 74 kDa nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673 amino acid protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc-finger domain. PLZF forms homodimers with RAR alpha and LAZ3 with its zinc-finger region. Alternate splice forms exist which are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. Human PLZF shares 96%, 97%, 96%, and 96% amino acid identity with rat, mouse, canine, and bovine PLZF, respectively.

Long Name
Promyelocytic Leukemia Zinc Finger
Entrez Gene IDs
7704 (Human); 235320 (Mouse)
Alternate Names
PLZF; PLZFzinc finger and BTB domain-containing protein 16; ZBTB16; zinc finger and BTB domain containing 16; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); Zinc finger protein 145; Zinc finger protein PLZF; ZNF145; ZNF145Promyelocytic leukemia zinc finger protein

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Citations for Human PLZF APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Culture, expansion, and flow-cytometry-based functional analysis of pteropid bat MR1-restricted unconventional T cells
    Authors: Wan Rong Sia, Ying Ying Hey, Randy Foo, Lin-Fa Wang, Edwin Leeansyah
    STAR Protocols
  2. RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses
    Authors: Todd Bradley, Dimitra Peppa, Isabela Pedroza-Pacheco, Dapeng Li, Derek W. Cain, Ricardo Henao et al.
    Cell
  3. MAIT cells are reduced in frequency and functionally impaired in human T lymphotropic virus type 1 infection: Potential clinical implications
    Authors: D Paquin-Pro, BC Greenspun, EA Costa, AC Segurado, EG Kallas, DF Nixon, FE Leal
    PLoS ONE, 2017-04-06;12(4):e0175345.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. TLR-induced cytokines promote effective proinflammatory natural Th17 cell responses.
    Authors: Massot B, Michel M, Diem S, Ohnmacht C, Latour S, Dy M, Eberl G, Leite-de-Moraes M
    J Immunol, 2014-05-07;192(12):5635-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Acquisition of innate-like microbial reactivity in mucosal tissues during human fetal MAIT-cell development.
    Authors: Leeansyah E, Loh L, Nixon D, Sandberg J
    Nat Commun, 2014-01-01;5(0):3143.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  6. Activation, exhaustion, and persistent decline of the antimicrobial MR1-restricted MAIT-cell population in chronic HIV-1 infection.
    Authors: Leeansyah E, Ganesh A, Quigley M, Sonnerborg A, Andersson J, Hunt P, Somsouk M, Deeks S, Martin J, Moll M, Shacklett B, Sandberg J
    Blood, 2012-12-13;121(7):1124-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Human iNKT and MAIT cells exhibit a PLZF-dependent proapoptotic propensity that is counterbalanced by XIAP.
    Authors: Gerart S, Siberil S, Martin E, Lenoir C, Aguilar C, Picard C, Lantz O, Fischer A, Latour S
    Blood, 2012-12-06;121(4):614-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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