Human PKC zeta Antibody Summary
Ile454-Val592
Accession # Q05513
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human PKC zeta by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, MCF-7 human breast cancer cell line, and human lung tissue. PVDF Membrane was probed with 1 µg/mL of Human PKC zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6666) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PKC zeta at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Western Blot Shows Human PKC zeta Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and PKC zeta knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PKC zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6666) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PKC zeta at approximately 75 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human PKC zeta by Simple WesternTM. Simple Western shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.5 mg/ml. A specific band was detected for PKC zeta at approximately 79 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human PKC zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6666). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKC zeta
PKC zeta (protein kinase C-zeta, EC=2.7.11.13), also called PKC2, is a 592 amino acid (aa), 79 kDa member of the atypical PKC (aPKC) subfamily of Ser/Thr protein kinases. aPKC proteins can be activated by phorbol esters, but are insensitive to calcium and diacylglycerol. PKC zeta regulates cell polarity in early development, and is necessary for cytokine-induced chemotaxis of macrophages and cancer cells, chemokine-induced adhesion of neutrophils, and polarization of Th2 cells. It is activated by PDK1 phosphorylation, binds ceramide, and translocates to the plasma membrane where it regulates adherens and tight junction formation. PKC zeta contains an OPR domain (aa 15-98), a zinc finger region (aa 130-180), and a protein kinase domain (aa 252-518). PKC zeta potential variants of 409 or 539 aa have alternate start sites at aa 184 or aa 9 (also missing aa 186-233), respectively. Within the region used as an immunogen, human PKC zeta shares 98% aa identity with mouse and rat PKC zeta.
Product Datasheets
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