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Human PILR-alpha Antibody

Catalog #: AF6484 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6484
AF6484-SP
Detection of PILR‑ alpha in Human peripheral blood granulocytes by Flow Cytometry.
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Human PILR-alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human PILR-alpha in direct ELISAs.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PILR-alpha
Gln20-Thr196
Accession # Q9UKJ1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of PILR-a antibody in Human peripheral blood granulocytes antibody by Flow Cytometry. View Larger

Detection of PILR‑ alpha in Human peripheral blood granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Sheep Anti-Human PILR-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6484, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PILR-alpha

PILR-alpha (paired immunoglobulin-like, type 2 receptor alpha) is a 44-50 kDa type I transmembrane (TM) paired receptor glycoprotein that belongs to the Ig Superfamily. It is expressed by monocytes, macrophages, CD14+CD1a- DC and retinal pigment cells, and is known to bind to CD99 and PANP. PILR-alpha acts as a receptor for HSV and serves as a negative immunomodulator that contains an ITIM. Mature human PILR-alpha is 284 amino acids (aa) in length. It contains one V-type Ig-like domain in its extracellular region (aa 32-150), and two ITIMs in its cytoplasmic domain (aa 267-272 and 296-301). There are multiple potential splice variants. One is TM and possesses a 35 aa substitution for aa 264-303, while others are soluble, and show a deletion of aa 152-224 that may be coupled to the 35 aa substitution noted above, or simply exhibit a 24 aa substitution for aa 152-303. Over aa 20-196, human PILR-alpha shares only 42% aa identity with mouse PILR-alpha, and 89% aa identity with human PILR-beta.

Long Name
Paired-Ig-likeType 2 Receptor alpha
Entrez Gene IDs
29992 (Human); 231805 (Mouse); 288568 (Rat)
Alternate Names
Cell surface receptor FDF03; FDF03; inhibitory receptor PILRalpha; Inhibitory receptor PILR-alpha; paired immunoglobin-like receptor alpha; paired immunoglobin-like type 2 receptor alpha; paired immunoglobulin-like receptor alpha; paired immunoglobulin-like type 2 receptor alpha; PILRA; PILRalpha; PILR-alpha

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