Human PIK3R5 Antibody

Catalog #: MAB6388 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6388
MAB6388-SP

Detection of Human PIK3R5 by Western Blot.

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Human PIK3R5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human PIK3R5 in Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 601214

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PIK3R5
Lys530-Pro726
Accession # Q8WYR1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PIK3R5 by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human PIK3R5 Monoclonal Antibody (Catalog # MAB6388) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for PIK3R5 at approximately 97 kDa and the spliced isoform at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PIK3R5

Phosphoinositide 3-kinase regulatory subunit 5 (PIK3R5; also PI3-kinase p101 subunit, Ptdlns-3-kinase p101, and p101-PI3K) is a 97 kDa regulatory subunit of the PI3K gamma complex. It is expressed as a heterodimer with the catalytic subunit PIK3CG/p120. Human PIK3R5 is 880 amino acids (aa) in length. The heterodimerization region is made up of aa 25-101, and aa 653-753 comprise the region for interaction with G beta gamma proteins. A second 55 kDa isoform is formed by the deletion of aa 1-386. Human PIK3R5 is 86% aa identical to mouse PIK3R5. PIK3R5 is highly expressed in leukocytes, followed by spleen, lymph node, thymus, and bone marrow.

Long Name

Phosphoinositide-3-kinase, Regulatory Subunit 5

Entrez Gene IDs

23533 (Human); 320207 (Mouse); 497931 (Rat)

Alternate Names

F730038I15Rik; FOAP-2; p101; p101-PI3K; Phosphatidylinositol-4,5-bisphosphate 3-kinase regulatory subunit; phosphoinositide-3-kinase, regulatory subunit 5; phosphoinositide-3-kinase, regulatory subunit, polypeptide p101; PI3-kinase p101 subunit; PI3-kinase regulatory subunit 5; PIK3R5; PtdIns-3-kinase regulatory subunit; regulatory subunit 5, p101

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