Human Phospho-STAT3 (Y705) Antibody

Catalog #: MAB46071
2 Citations Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB46071-100
MAB46071-SP

Detection of Human Phospho-STAT3 (Y705) by Western Blot.

3 Images

All STAT3 Antibodies

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-STAT3 (Y705) Antibody Summary

Species Reactivity

Human

Specificity

Detects human STAT3 when phosphorylated at Y705 in Western blots.

Source

Recombinant Monoclonal Rabbit IgG_2B Clone # 1004G

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Phosphopeptide containing the human STAT3 Y705 site
Accession # P40763

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Simple Western

2 µg/mL

Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 50 µg/ml Recombinant Human IL-22 (Catalog # 782-IL) for 15 minutes

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human Phospho-STAT3 (Y705) by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-22 (Catalog # 782-IL) for 15 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-STAT3 (Y705) Monoclonal Antibody (Catalog # MAB46071) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-STAT3 (Y705) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry

Detection of Phospho-STAT3 (Y705) antibody in IFN alpha-treated Daudi Human Cell Line antibody by Flow Cytometry.

View Larger

Detection of Phospho-STAT3 (Y705) in IFN alpha-treated Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was unstimulated (open histogram) or treated with 500 U/mL rhIFN-alpha for 20 minutes (filled histogram) and stained with Rabbit anti-Human Phospho-STAT3 (Y705) Molyclonal Antibody (Catalog # MAB46071) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

Simple Western

View Larger

Detection of Human STAT3 by Simple Western^TM. Simple Western shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 50 µg/ml Recombinant Human IL‑22 (Catalog # 782-IL) for 15 minutes, loaded at 0.2 mg/ml. A specific band was detected for STAT3 at approximately 89 kDa (as indicated) using 2 µg/mL of Rabbit Anti-Human Phospho-STAT3 (Y705) Monoclonal Antibody (Catalog # MAB46071). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT3

Human STAT-3/STAT3a (signal transducer and activator of transcription 3; also called APRF) is a 90-95 kDa member of the STAT family of transcription factors. It is 770 amino acids (aa) in length, contains one SH2 domain (aa 580-670), and is found in almost all cell types. STAT3 mediates gp130, LIF-R, OB-R, IL10-R and EGFR signaling. Upon activation, receptors such as gp130 phosphorylate STAT3 at Ser727, which results in transcriptional activation. Phosphorylation at Tyr705 mediates homo- and hetero-dimerization with STAT1 and nuclear translocation, and is an important event in oncogenic transformation. The STAT3b isoform shows a six aa substitution for aa 716-770, thus eliminating the Ser727 site. Human and mouse STAT3a share 99% aa sequence identity.

Long Name

Signal Transducer and Activator of Transcription 3

Entrez Gene IDs

6774 (Human); 20848 (Mouse); 25125 (Rat)

Alternate Names

Acute-phase response factor; APRFMGC16063; DNA-binding protein APRF; FLJ20882; HIES; signal transducer and activator of transcription 3 (acute-phase responsefactor); signal transducer and activator of transcription 3; STAT3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Phospho-STAT3 (Y705) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

Human omental adipose-derived mesenchymal stem cells enhance autophagy in ovarian carcinoma cells through the STAT3 signalling pathway
Authors: Y Chu, Y Wang, K Li, M Liu, Y Zhang, Y Li, X Hu, C Liu, H Zhou, J Zuo, W Peng
Cell. Signal., 2020-01-24;69(0):109549.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Adipose-derived mesenchymal stem cells promote osteosarcoma proliferation and metastasis by activating the STAT3 pathway
Authors: Y Wang, Y Chu, B Yue, X Ma, G Zhang, H Xiang, Y Liu, T Wang, X Wu, B Chen
Oncotarget, 2017-04-04;8(14):23803-23816.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot