Human Phospho-p38 alpha (T180/Y182) Antibody Summary
Accession # Q16539
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Phospho-p38 alpha (T180/Y182) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 20 mJ/cm2ultraviolet light (UV) followed by a 30 minute recovery. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-p38a (T180/Y182) Monoclonal Antibody (Catalog # MAB8691) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-p38a (T180/Y182) at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p38 alpha
The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases activated by proinflammatory cytokines and environmental stresses. All four p38 family members, alpha, beta, gamma, and delta, are phosphorylated by MKK3 and/or MKK6 at dual Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr. Once activated, p38 phosphorylates a number of targets, including the nuclear transcription factors ATF2 and Max.
The most frequently analyzed family member, p38 alpha, also known as SAPK2a and MAPK14, was initially purified as a kinase critical to the signaling cascade linking IL-1 to MAPKAPK-2 and the small heat shock protein HSP27. Ubiquitously expressed, p38 alpha is dually phosphorylated by MKK3 and MKK6 at Thr180 and Tyr182. Once activated, p38 alpha phosphorylates a number of targets, including the cytoplasmic kinases MNK 4 and PRAK5 and the nuclear transcription factors ATF2 1 and STAT1. Several promising compounds that inhibit p38 alpha are being investigated as potential therapies for arthritic and inflammatory diseases.
Product Datasheets
Citations for Human Phospho-p38 alpha (T180/Y182) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Protective Effects of Lactic Acid Bacteria Against TLR4 Induced Inflammatory Response in Hepatoma HepG2 Cells Through Modulation of Toll-Like Receptor Negative Regulators of Mitogen-Activated Protein Kinase and NF-kappa B Signaling
Authors: Paulraj Kanmani, Hojun Kim
Frontiers in Immunology
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Combined anticancer therapy with imidazoacridinone C-1305 and paclitaxel in human lung and colon cancer xenografts-Modulation of tumour angiogenesis
Authors: M ?witalska, B Filip-Psur, M Milczarek, M Psurski, A Moszy?ska, AM D?browska, M Gawro?ska, K Krzymi?ski, M Bagi?ski, R Bartoszews, J Wietrzyk
Oncogene, 2022-06-14;0(0):.
Species: Xenograft
Sample Types: Tissue Homogenates
Applications: Simple Western -
Pharmacological targeting of the novel &beta-catenin chromatin-associated kinase p38&alpha in colorectal cancer stem cell tumorspheres and organoids
Authors: M Lepore Sig, V Grossi, S Di Franco, G Forte, V Disciglio, C Fasano, P Sanese, K De Marco, FC Susca, LR Mangiapane, A Nicotra, G Di Carlo, F Dituri, G Giannelli, G Ingravallo, G Canettieri, G Stassi, C Simone
Cell Death & Disease, 2021-03-25;12(4):316.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Immunobiotics Beneficially Modulate TLR4 Signaling Triggered by Lipopolysaccharide and Reduce Hepatic Steatosis In Vitro
Authors: P Kanmani, A Ansari, J Villena, H Kim
J Immunol Res, 2019-03-14;2019(0):3876896.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The p38? Stress Kinase Suppresses Aneuploidy Tolerance by Inhibiting Hif-1?
Authors: S Simões-Sou, S Littler, SL Thompson, P Minshall, H Whalley, B Bakker, K Belkot, D Moralli, D Bronder, A Tighe, DCJ Spierings, N Bah, J Graham, L Nelson, CM Green, F Foijer, PA Townsend, SS Taylor
Cell Rep, 2018-10-16;25(3):749-760.e6.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Up-regulation of NHE8 by somatostatin ameliorates the diarrhea symptom in infectious colitis mice model
Authors: X Lei, L Cai, X Li, H Xu, C Geng, C Wang
Korean J. Physiol. Pharmacol., 2018-04-25;22(3):269-275.
Species: Human, Mouse
Sample Types: Cell Lysates, Tissue Homogenates
Applications: Western Blot -
IL17A augments autophagy in Mycobacterium tuberculosis-infected monocytes from patients with active tuberculosis in association with the severity of the disease
Authors: NL Tateosian, JM Pellegrini, NO Amiano, A Rolandelli, N Casco, DJ Palmero, MI Colombo, VE García
Autophagy, 2017-06-05;0(0):14-Jan.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Regulatory module involving FGF13, miR-504, and p53 regulates ribosomal biogenesis and supports cancer cell survival
Proc. Natl. Acad. Sci. U.S.A, 2016-12-19;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Photoprotective Effects of Cycloheterophyllin against UVA-Induced Damage and Oxidative Stress in Human Dermal Fibroblasts
PLoS ONE, 2016-09-01;11(9):e0161767.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cynandione�A inhibits lipopolysaccharide-induced cell adhesion via suppression of the protein expression of VCAM‑1 in human endothelial cells
Authors: Keun Hyung Park, Jiyoung Kim, Eunjoo H Lee, Tae Hoon Lee
International Journal of Molecular Medicine
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