Human Phospho-MSK1 (S376)/MSK2 (S360) Antibody

Catalog #: AF1094
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1094
AF1094-SP

Detection of Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) by Western Blot.

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-MSK1 (S376)/MSK2 (S360) Antibody Summary

Species Reactivity

Human

Specificity

Detects human MSK1 and MSK2 when phosphorylated at S376 and S360, respectively. The amino acid sequence surrounding S360 of mouse MSK2 is identical to the phosphopeptide antigen.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing human MSK1 S376 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line untreated (-) or treated (+) with 200 ng/mL Recombinant Human EGF (Catalog # 236-EG) in the presence or absence of pathway inhibitors SB203580 and PD98059 for 20 minutes. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSK1/MSK2

Mitogen- and stress-activated protein kinase 1 (MSK1), also known as ribosomal protein S6 kinase 5 (RPS6KA5), belongs to the AGC family of kinases. Both MSK1 and the related MSK2, also known as RSKB and RPS6KA4, have two kinase domains connected by a regulatory linker region, and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB.

Long Name

Mitogen- and Stress-activated Protein Kinase 1 and 2

Alternate Names

1110069D02Rik; 3110005L17Rik; 6330404E13Rik; MSK1/MSK2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Phospho-MSK1 (S376)/MSK2 (S360) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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