Human Pentraxin 3/TSG-14 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY1826
DY1826-05
Ancillary Products Available
Human Pentraxin 3 / TSG-14 ELISA Standard Curve
1 Image
Product Details
Procedure
Citations (13)
FAQs
Supplemental Products
Reviews (1)

Human Pentraxin 3/TSG-14 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
218.0 - 14,000 pg/mL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Pentraxin 3. The suggested diluent is suitable for the analysis of most cell culture supernate, serum, and plasma samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

Human Pentraxin 3 / TSG-14 ELISA Standard Curve

Product Datasheets

You must select a language.

x

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Pentraxin 3/TSG-14

Pentraxin 3 (PTX3), also known as TNF-stimulated gene 14 (TSG-14), is a secreted glycoprotein belonging to the Pentraxin family of proteins. It is the prototypical long Pentraxin, exhibiting a C-terminal Pentraxin domain characteristic of the family, and a unique N-terminal domain. It is produced by several cell types including endothelial and mononuclear cells. PTX3 acts as a pattern recognition receptor with non-redundant roles in the innate immune response to several microbial agents including the fungal pathogen Aspergillus fumigatus. Overexpression leads to enhanced pro-inflammatory responses. PTX3 is induced in response to LPS and inflammatory cytokines including TNF-alpha and IL-1 beta.

Long Name:
Tumor Necrosis Factor Stimulated Gene 14
Entrez Gene IDs:
5806 (Human); 19288 (Mouse)
Alternate Names:
alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; Pentraxin 3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

 

Citations for Human Pentraxin 3/TSG-14 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Pentraxin 3 and Shigella LPS and IpaB Antibodies Interplay to Defeat Shigellosis
    Authors: S Meron-Suda, A Reizis, S Goren, A Bialik, A Hochberg, D Cohen
    Journal of Clinical Medicine, 2022-07-28;11(15):.
    Species: Human
    Sample Types: Serum
  2. Prediction of emergency cerclage outcomes in women with cervical insufficiency: The role of inflammatory, angiogenic, and extracellular matrix-related proteins in amniotic fluid
    Authors: KN Lee, KH Park, YM Kim, I Cho, TE Kim
    PLoS ONE, 2022-05-10;17(5):e0268291.
    Species: Human
    Sample Types: Amniotic Fluid
  3. Oxidative Stress-Induced Pentraxin 3 Expression Human Retinal Pigment Epithelial Cells is Involved in the Pathogenesis of Age-Related Macular Degeneration
    Authors: N Hwang, MY Kwon, JM Woo, SW Chung
    Int J Mol Sci, 2019-11-29;20(23):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Loss of skin elasticity is associated with pulmonary emphysema, biomarkers of inflammation, and matrix metalloproteinase activity in smokers
    Authors: ME O'Brien, D Chandra, RC Wilson, CM Karoleski, CR Fuhrman, JK Leader, J Pu, Y Zhang, A Morris, S Nouraie, J Bon, Z Urban, FC Sciurba
    Respir. Res., 2019-06-24;20(1):128.
    Species: Human
    Sample Types: Plasma
  5. Calcineurin inhibitors reduce NFAT-dependent expression of antifungal pentraxin-3 by human monocytes
    Authors: K Bendí?ková, F Tidu, M De Zuani, MH Kohoutková, I Andrej?ino, A Pompeiano, S B?lášková, G Forte, T Zelante, J Fri?
    J. Leukoc. Biol., 2019-04-01;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. The Long Pentraxin 3 Contributes to Joint Inflammation in Gout by Facilitating the Phagocytosis of Monosodium Urate Crystals
    Authors: NV Batista, M Barbagallo, VLS Oliveira, T Castro-Gom, RDR Oliveira, P Louzada-Ju, GRC Pinheiro, A Mantovani, MM Teixeira, C Garlanda, FA Amaral
    J. Immunol., 2019-02-04;0(0):.
    Species: Human
    Sample Types: Plasma
  7. Role of a fluid-phase PRR in fighting an intracellular pathogen: PTX3 in Shigella infection
    Authors: V Ciancarell, L Lembo-Fazi, I Paciello, AK Bruno, S Jaillon, S Berardi, M Barbagallo, S Meron-Suda, D Cohen, A Molinaro, G Rossi, C Garlanda, ML Bernardini
    PLoS Pathog., 2018-12-07;14(12):e1007469.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Serum Pentraxin 3 is associated with signs of arterial alteration in women with preeclampsia
    Authors: T Akhter, AK Wikström, M Larsson, A Larsson, G Wikström, T Naessen
    Int. J. Cardiol., 2017-03-24;0(0):.
    Species: Human
    Sample Types: Serum
  9. Tunicamycin-induced Endoplasmic Reticulum Stress Upregulates the Expression of Pentraxin 3 in Human Retinal Pigment Epithelial Cells
    Authors: Narae Hwang
    Korean J Ophthalmol, 2016-12-06;30(6):468-478.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Role of pentraxin 3 in shaping arthritogenic alphaviral disease: from enhanced viral replication to immunomodulation.
    Authors: Foo, Suan-Sin, Chen, Weiqiang, Taylor, Adam, Sheng, Kuo-Chin, Yu, Xing, Teng, Terk-Shi, Reading, Patrick, Blanchard, Helen, Garlanda, Cecilia, Mantovani, Alberto, Ng, Lisa F P, Herrero, Lara J, Mahalingam, Suresh
    PLoS Pathog, 2015-02-19;11(2):e1004649.
    Species: Human
    Sample Types: Serum
  11. Severe Acinetobacter baumannii sepsis is associated with elevation of pentraxin 3.
    Authors: Ketter P, Guentzel M, Schaffer B, Herzig M, Wu X, Montgomery R, Parida B, Fedyk C, Yu J, Jorgensen J, Chambers J, Cap A, Arulanandam B
    Infect Immun, 2014-07-07;82(9):3910-8.
    Species: Mouse
    Sample Types: Serum
  12. Inflammatory biomarker pentraxin 3 (PTX3) in relation to obesity, body fat depots and weight loss.
    Authors: Witasp A, Carrero J, Michaelsson K, Ahlstrom H, Kullberg J, Adamsson V, Riserus U, Larsson A, Helmersson-Karlqvist J, Lind L, Stenvinkel P, Arnlov J
    Obesity (Silver Spring), 2014-01-25;22(5):1373-9.
    Species: Human
    Sample Types: Serum
  13. Exogenous pentraxin 3 restores antifungal resistance and restrains inflammation in murine chronic granulomatous disease.
    Authors: D'Angelo C, D'Angelo C, De Luca A, Zelante T, Bonifazi P, Moretti S, Giovannini G, Iannitti RG, Zagarella S, Bozza S, Campo S, Salvatori G, Romani L
    J. Immunol., 2009-09-04;183(7):4609-18.
    Species: Human
    Sample Types: Tissue Homogenates

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs
Loading...

Reviews for Human Pentraxin 3/TSG-14 DuoSet ELISA

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human Pentraxin 3/TSG-14 DuoSet ELISA?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human Pentraxin 3/TSG-14 DuoSet ELISA
By Anonymous on 07/14/2017
Sample Tested: Human serum