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Human PDGF R alpha Antibody

Catalog #: MAB322
20 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB322-SP
MAB322-100
MAB322-500
Cell Proliferation Induced by PDGF‑AA and Neutralization by Human PDGF R alpha Antibody.
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Product Details
Citations (20)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PDGF R alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human PDGF R beta or recombinant mouse PDGF R alpha is observed.
Source
Monoclonal Mouse IgG1 Clone # 35248
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PDGF R alpha
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human PDGF R alpha (Catalog # 322-PR) under non-reducing conditions only
Immunohistochemistry
8-25 µg/mL
See below
Neutralization
Measured by its ability to neutralize PDGF‑AA-induced proliferation in the WS‑1 human fetal skin fibroblast cell line. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 10 ng/mL Recombinant Human PDGF‑AA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by PDGF‑AA and Neutralization by Human PDGF R alpha Antibody. View Larger

Cell Proliferation Induced by PDGF‑AA and Neutralization by Human PDGF R alpha Antibody. Recombinant Human PDGF-AA (Catalog # 221-AA) stimulates proliferation in the WS-1 human fetal skin fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-AA (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human PDGF Ra Monoclonal Antibody (Catalog # MAB322). The ND50 is typically 2-10 µg/mL.

Immunohistochemistry PDGF Ra antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

PDGF R alpha in Human Breast Cancer Tissue. PDGF Ra was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human PDGF Ra Monoclonal Antibody (Catalog # MAB322) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF R alpha

PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF‑BB and AB, but not PDGF-AA. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Only PDGF R alpha is expressed in oligodendrocyte progenitor cells, mesothelial cell and liver endothelial cells. Soluble PDGF-R alpha has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF R alpha binds PDGF with high affinity and is a potent PDGF antagonist (1).

References
  1. Heldin, C.H. and L. Claesson-Welsh (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A. (ed) Oxford University Press, New York, NY p. 202.
Long Name
Platelet-derived Growth Factor Receptor alpha
Entrez Gene IDs
5156 (Human); 18595 (Mouse)
Alternate Names
alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA

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Citations for Human PDGF R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. A phosphorylation switch controls the spatiotemporal activation of Rho GTPases in directional cell migration
    Authors: Xuan Cao, Tomonori Kaneko, Jenny S. Li, An-Dong Liu, Courtney Voss, Shawn S. C. Li
    Nature Communications
  2. Intrinsic regulation of hemangioma involution by platelet-derived growth factor
    Authors: E E Roach, R Chakrabarti, N I Park, E C Keats, J Yip, N G Chan et al.
    Cell Death & Disease
  3. Human Cardiac-Mesenchymal Stem Cell-Like Cells, a Novel Cell Population with Therapeutic Potential
    Authors: Rachel Oldershaw, W. Andrew Owens, Rachel Sutherland, Martin Linney, Rachel Liddle, Lissette Magana et al.
    Stem Cells and Development
  4. Characterization of binding and quantification of human autoantibodies to PDGFRa using a biosensor-based approach.
    Authors: Moroncini G, Cuccioloni M, Mozzicafreddo M et al.
    Anal Biochem
  5. Epigenetic Modulation of Human Induced Pluripotent Stem Cell Differentiation to Oligodendrocytes
    Authors: Panagiotis Douvaras, Tomasz Rusielewicz, Kwi Hye Kim, Jeffery D. Haines, Patrizia Casaccia, Valentina Fossati
    International Journal of Molecular Sciences
  6. Platelet?derived growth factor receptor?? gene expression relates to recurrence in colorectal cancer
    Authors: S Fujino, N Miyoshi, M Ohue, Y Takahashi, M Yasui, T Hata, C Matsuda, T Mizushima, Y Doki, M Mori
    Oncol. Rep., 2018-03-01;0(0):.
  7. Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling
    Authors: ST Wei, YC Huang, ML Hsieh, YJ Lin, WC Shyu, HC Chen, CH Hsieh
    Cell Death Dis, 2020-05-04;11(5):307.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  8. Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-? as a key for entry
    Authors: Y Wu, A Prager, S Boos, M Resch, I Brizic, M Mach, S Wildner, L Scrivano, B Adler
    PLoS Pathog., 2017-04-12;13(4):e1006281.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Structural environment built by AKAP12+ colon mesenchymal cells drives M2 macrophages during inflammation recovery
    Authors: JM Yang, HS Lee, JH Seo, JH Park, IH Gelman, EH Lo, KW Kim
    Sci Rep, 2017-02-16;7(0):42723.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Fractional CO2 laser: a novel therapeutic device upon photobiomodulation of tissue remodeling and cytokine pathway of tissue repair.
    Authors: Prignano F, Campolmi P, Bonan P
    Dermatol Ther, 2009-11-01;22(0):S8-15.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines.
    Authors: Li R, Maminishkis A, Wang FE, Miller SS
    Invest. Ophthalmol. Vis. Sci., 2007-12-01;48(12):5722-32.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Neutralization, Western Blot
  12. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007-04-30;177(3):489-500.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Interleukin-11 potentiates oligodendrocyte survival and maturation, and myelin formation.
    Authors: Zhang Y, Taveggia C, Melendez-Vasquez C, Einheber S, Raine CS, Salzer JL, Brosnan CF, John GR
    J. Neurosci., 2006-11-22;26(47):12174-85.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. Co-expression of PDGF alpha receptor and NG2 by oligodendrocyte precursors in human CNS and multiple sclerosis lesions.
    Authors: Wilson HC, Scolding NJ, Raine CS
    J. Neuroimmunol., 2006-06-06;176(1):162-73.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  15. Heterodimerization of FGF-receptor 1 and PDGF-receptor-alpha: a novel mechanism underlying the inhibitory effect of PDGF-BB on FGF-2 in human cells.
    Authors: Faraone D, Aguzzi MS, Ragone G, Russo K, Capogrossi MC, Facchiano A
    Blood, 2005-12-01;107(5):1896-902.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. In vitro cytotoxic effects of imatinib in combination with anticancer drugs in human prostate cancer cell lines.
    Authors: Kubler HR, van Randenborgh H, Treiber U, Wutzler S, Battistel C, Lehmer A, Wagenpfeil S, Hartung R, Paul R
    Prostate, 2005-06-01;63(4):385-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
    Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
    J. Cell. Physiol., 2005-04-01;203(1):35-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Metabotropic glutamate receptors are expressed in adult human glial progenitor cells.
    Authors: Luyt K, Varadi A, Halfpenny CA, Scolding NJ, Molnar E
    Biochem. Biophys. Res. Commun., 2004-06-18;319(1):120-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. Myristoylated Alanine‐Rich Protein Kinase Substrate (MARCKS) Regulates Small GTPase Rac1 and Cdc42 Activity and Is a Critical Mediator of Vascular Smooth Muscle Cell Migration in Intimal Hyperplasia Formation
    Authors: Dan Yu, George Makkar, Dudley K. Strickland, Thomas A. Blanpied, Deborah J. Stumpo, Perry J. Blackshear et al.
    Journal of the American Heart Association
  20. SOX11 promotes tumor angiogenesis through transcriptional regulation of PDGFA in mantle cell lymphoma
    Authors: Jara Palomero, Maria Carmela Vegliante, Marta Leonor Rodríguez, Álvaro Eguileor, Giancarlo Castellano, Ester Planas-Rigol et al.
    Blood

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Reviews for Human PDGF R alpha Antibody

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Human PDGF R alpha Antibody
By Anonymous on 09/13/2021
Application: IHC Sample Tested: Ovary tissue Species: Human
Human PDGF R alpha Antibody
By Anonymous on 12/01/2017
Application: ELISA Sample Tested: Purified Standard Species: Human