Home / PDGF-C / Human PDGF-C Antibody

Human PDGF-C Antibody

Catalog #: MAB1560
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1560-SP
MAB1560-100
MAB1560-500
Proliferation Induced by PDGF‑CC and Neutralization by Human PDGF‑C Antibody.
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PDGF-C Antibody Summary

Species Reactivity
Human
Specificity
Detects human PDGF-C in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse PDGF-CC is observed, and no cross-reactivity with recombinant human (rh) PDGF-AA, rhPDGF-AB, rhPDGF-BB, rhPDGF-D, or recombinant rat PDGF-AB is observed.
Source
Monoclonal Mouse IgG2B Clone # 619346
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E.coli-derived recombinant human PDGF-C
Val235-Gly345
Accession # Q9NRA1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize PDGF‑CC -induced proliferation in the NR6R‑3T3 mouse fibroblast cell line [Raines, E.W. et al. (1985) Methods Enzymol. 109:749]. The Neutralization Dose (ND50) is typically 0.6-3 ug/ml in the presence of 1 ug/mL Recombinant Human PDGF‑C (Catalog # 1687-CC).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Proliferation Induced by PDGF‑CC and Neutralization by Human PDGF‑C Antibody. View Larger

Proliferation Induced by PDGF‑CC and Neutralization by Human PDGF‑C Antibody. Recombinant Human PDGF-CC (Catalog # 1687-CC) induces proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human PDGF-CC (1 ug/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human PDGF-C Monoclonal Antibody (Catalog # MAB1560). The ND50 is typically 0.6-3 ug/ml.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-C

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form four disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and one heterodimer (PDGF-AB) (1). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero‑dimerization of two receptor tyrosine kinases (PDGF R alpha  and R beta ). Within the PDGF family, PDGF-C and PDGF-D constitute a subgroup that shares similar structural organization (2, 3). Both proteins are secreted as inactive homodimeric latent growth factors. Each monomer has two distinct protein domains: an N-terminal CUB domain; and a C-terminal PDGF/VEGF homology domain that shares 27‑35% sequence identity with the corresponding regions of other PDGF family members. An 80‑90 amino acid residue hinge region connects the two domains. Sequential removal of the CUB domains in the homodimeric latent growth factor by extracellular proteolytic cleavage at the hinge region is required to release the bioactive PDGF/VEGF homology domain(1). Twelve cysteine residues are found within the PDGF/VEGF homology domain of PDGF-C, including the characteristic eight invariant cysteine residues involved in inter- and intra-chains disulfide-bonds needed for the formation of the cysteine-knot structure. Bioactive PDGF-CC binds with high-affinity to PDGF R alpha  but not PDGF R beta  and activates PDGF R alpha homodimerization (1). PDGF-CC has also been shown to activate PDGF R alpha beta heterodimers (1). PDGF-CC is expressed in multiple embryonic and adult cell types and tissues. During embryonic development, PDGF-CC is involved in ductal morphogenesis (4). PDGF-CC is a potent angiogenic factor that stimulates vessel growth in the mouse cornea pocket assay and in the CAM assay (5). It stimulates coronary artery smooth muscle cell proliferation and may play an important role in cardiovascular development and function (6). PDGF-CC is also expressed in many tumors and tumor cell lines and has a causative role in tumorigenesis (7). Mature human and mouse PDGF-C share 93.7% amino acid sequence identity.

References
  1. Li, X. and U. Eriksson (2003) Cytokine &Growth Factor Rev. 14:91.
  2. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.
  3. Li, X. et al. (2000) Nature Cell Biol. 2:302.
  4. Aase, K. et al. (2002) Mech Dev. 110:187.
  5. Cao, R.H. et al. (2002) FASEB J. 16:1575.
  6. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
  7. Zwerner, J.P. and W.A. May (2001) Oncogene 20:626.
Long Name
Platelet-derived Growth Factor C
Entrez Gene IDs
56034 (Human); 54635 (Mouse)
Alternate Names
FALLOTEIN; PDGFC; PDGF-C; platelet derived growth factor C; platelet-derived growth factor C; SCDGFSpinal cord-derived growth factor; secretory growth factor-like protein; VEGF-E

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human PDGF-C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Vascular immunotargeting to endothelial determinant ICAM-1 enables optimal partnering of recombinant scFv-thrombomodulin fusion with endogenous cofactor.
    Authors: Greineder, Colin F, Chacko, Ann-Mari, Zaytsev, Sergei, Zern, Blaine J, Carnemolla, Ronald, Hood, Elizabet, Han, Jingyan, Ding, Bi-Sen, Esmon, Charles, Muzykantov, Vladimir
    PLoS ONE, 2013-11-14;8(11):e80110.
  2. Lifelong choline supplementation ameliorates Alzheimer's disease pathology and associated cognitive deficits by attenuating microglia activation.
    Authors: Velazquez R, Ferreira E et al.
    Aging Cell

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human PDGF-C Antibody

There are currently no reviews for this product. Be the first to review Human PDGF-C Antibody and earn rewards!

Have you used Human PDGF-C Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review