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Human PDGF-BB DuoSet ELISA

Catalog #: DY220
25 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY220
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
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Human PDGF-BB ELISA Standard Curve
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Product Details
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Citations (25)
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human PDGF-BB DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
31.2 - 2,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human PDGF-BB. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Human PDGF-BB ELISA Standard Curve View Larger

Human PDGF-BB ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PDGF-BB

The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).

Long Name:
Platelet-derived Growth Factor BB
Entrez Gene IDs:
5155 (Human); 24628 (Rat)
Alternate Names:
c-sis;IBGC5;PDGF2;PDGF-2;PDGFB;Platelet-derived growth factor subunit B;SIS;SSV; PDGFBB; PDGF-BB

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

 

Citations for Human PDGF-BB DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. Characterization of platelet rich plasma in feline immunodeficiency virus-infected cats: Cell, and PDGF-BB and TGF-beta 1 growth factor analysis
    Authors: Miguel-Pastor, L;Satué, K;Chicharro, D;Damiá, E;Cuervo, B;Torres-Torrillas, M;Martins, E;Velasco-Martínez, MG;Carrillo, JM;Sopena, JJ;Cerón, JJ;Rubio, M;
    Research in veterinary science
    Species: Feline
    Sample Types: Platelet-Poor Plasma, Platelet-Rich Plasma
  2. Hypoxia Preconditioned Serum (HPS) Promotes Proliferation and Chondrogenic Phenotype of Chondrocytes In Vitro
    Authors: Jiang, J;Altammar, J;Cong, X;Ramsauer, L;Steinbacher, V;Dornseifer, U;Schilling, AF;Machens, HG;Moog, P;
    International journal of molecular sciences
    Species: Human
    Sample Types: Serum
  3. Effectiveness of BMP-2 and PDGF-BB Adsorption onto a Collagen/Collagen-Magnesium-Hydroxyapatite Scaffold in Weight-Bearing and Non-Weight-Bearing Osteochondral Defect Bone Repair: In Vitro, Ex Vivo and In Vivo Evaluation
    Authors: J Xu, S Fahmy-Garc, MA Wesdorp, N Kops, L Forte, C De Luca, MM Misciagna, L Dolcini, G Filardo, M Labberté, K Vancíková, J Kok, B van Rietbe, J Nickel, E Farrell, PAJ Brama, GJVM van Osch
    Journal of functional biomaterials, 2023-02-16;14(2):.
    Species: Human
    Sample Types: Complex Sample Type
  4. In Vitro Comparison of Lymphangiogenic Potential of Hypoxia Preconditioned Serum (HPS) and Platelet-Rich Plasma (PRP)
    Authors: J Jiang, X Cong, S Alageel, U Dornseifer, AF Schilling, E Hadjipanay, HG Machens, P Moog
    International Journal of Molecular Sciences, 2023-01-19;24(3):.
    Species: Human
    Sample Types: Hypoxia Preconditioned Serum
  5. In situ regeneration of nasal septal defects using acellular cartilage enhanced with platelet-derived growth factor
    Authors: H Lena, G David, K Johann, J Yvonne, Z Frank G, H Daniela, B Roman, B Rolf E, R Nicole
    Journal of tissue engineering, 2022-09-20;13(0):2041731422111.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  6. Growth Factor Release within Liquid and Solid PRF
    Authors: K Zwittnig, B Kirnbauer, N Jakse, P Schlenke, I Mischak, S Ghanaati, S Al-Maawi, D Végh, M Payer, TA Zrnc
    Journal of Clinical Medicine, 2022-08-29;11(17):.
    Species: Human
    Sample Types: Serum
  7. Therapeutic Effects of Platelet-Derived Extracellular Vesicles in a Bioengineered Tendon Disease Model
    Authors: AL Graça, RMA Domingues, I Calejo, M Gómez-Flor, ME Gomes
    International Journal of Molecular Sciences, 2022-03-09;23(6):.
    Species: Human
    Sample Types: Extracellular Vesicles
  8. Circulating Interleukin-6 and CD16 positive monocytes increase following angioplasty of an arteriovenous fistula
    Authors: S Hakki, EJ Robinson, MG Robson
    Scientific Reports, 2022-01-26;12(1):1427.
    Species: Human
    Sample Types: Plasma
  9. Engagement of the CXCL12-CXCR4 Axis in the Interaction of Endothelial Progenitor Cell and Smooth Muscle Cell to Promote Phenotype Control and Guard Vascular Homeostasis
    Authors: SF Mause, E Ritzel, A Deck, F Vogt, EA Liehn
    International Journal of Molecular Sciences, 2022-01-14;23(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Intramammary treatment using allogeneic pure platelet-rich plasma in cows with subclinical mastitis caused by Gram-positive bacteria
    Authors: PC Duque-Madr, J Velasco-Bo, A Ceballos-M, C López, JU Carmona
    Scientific Reports, 2021-12-09;11(1):23737.
    Species: Bovine
    Sample Types: Plasma
  11. Why PRP works only on certain patients with tennis elbow? Is PDGFB gene a key for PRP therapy effectiveness? A prospective cohort study
    Authors: P Niemiec, K Szyluk, A Balcerzyk, M Kalita, A Jarosz, J Iwanicka, T Iwanicki, T Nowak, M Negru, T Francuz, W Garczorz, W Grzeszczak, S Górczy?ska, W Kania, I ?ak
    Bmc Musculoskeletal Disorders, 2021-08-18;22(1):710.
    Species: Human
    Sample Types: Plasma
  12. Regional hyperthermia enhances mesenchymal stem cell recruitment to tumor stroma: Implications for mesenchymal stem cell-based tumor therapy
    Authors: M Tutter, C Schug, KA Schmohl, S Urnauer, C Kitzberger, N Schwenk, M Petrini, C Zach, S Ziegler, P Bartenstei, WA Weber, G Multhoff, E Wagner, LH Lindner, PJ Nelson, C Spitzweg
    Mol Ther, 2020-10-15;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Standing wave-assisted acoustic droplet vaporization for single and dual payload release in acoustically-responsive scaffolds
    Authors: M Aliabouzar, A Jivani, X Lu, OD Kripfgans, JB Fowlkes, ML Fabiilli
    Ultrason Sonochem, 2020-03-26;66(0):105109.
    Species: N/A
    Sample Types: Complex Sample Type
  14. Ultrasonic Generation of Pulsatile and Sequential Therapeutic Delivery Profiles from Calcium-Crosslinked Alginate Hydrogels
    Authors: T Emi, K Michaud, E Orton, G Santilli, C Linh, M O'Connell, F Issa, S Kennedy
    Molecules, 2019-03-16;24(6):.
    Species: Human
    Sample Types: Hydrogel Fluid
  15. Long-term cytokine and growth factor release from equine platelet-rich fibrin clots obtained with two different centrifugation protocols
    Authors: RF Jiménez-Ar, C López, ME Álvarez, C Giraldo, M Prades, JU Carmona
    Cytokine, 2017-06-23;97(0):149-155.
    Species: Equine
    Sample Types: Plasma
  16. The PDGF-BB-SOX7 axis-modulated IL-33 in pericytes and stromal cells promotes metastasis through tumour-associated macrophages
    Nat Commun, 2016-05-06;7(0):11385.
    Species: Human
    Sample Types: Cell Lysates
  17. Regulating VEGF signaling in platelet concentrates via specific VEGF sequestering
    Authors: DG Belair, NN Le, WL Murphy
    Biomater Sci, 2016-03-24;4(5):819-25.
    Species: Human
    Sample Types: Plasma
  18. Effects of sodium citrate and acid citrate dextrose solutions on cell counts and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel.
    Authors: Giraldo C, Alvarez M, Carmona J
    BMC Vet Res, 2015-03-14;11(0):60.
    Species: Equine
    Sample Types: Plasma
  19. Effects of the breed, sex and age on cellular content and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel.
    Authors: Giraldo C, Lopez C, Alvarez M, Samudio I, Prades M, Carmona J
    BMC Vet Res, 2013-02-12;9(0):29.
    Species: Equine
    Sample Types: Plasma
  20. The 12th-14th type III repeats of fibronectin function as a highly promiscuous growth factor-binding domain.
    Authors: Martino MM, Hubbell JA
    FASEB J., 2010-07-29;24(12):4711-21.
    Species: Human
    Sample Types: Protein
  21. Improvement in disability after alemtuzumab treatment of multiple sclerosis is associated with neuroprotective autoimmunity.
    Authors: Jones JL, Anderson JM, Phuah CL, Fox EJ, Selmaj K, Margolin D, Lake SL, Palmer J, Thompson SJ, Wilkins A, Webber DJ, Compston DA, Coles AJ
    Brain, 2010-07-21;133(0):2232-47.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Normal ranges of angiogenesis regulatory proteins in human platelets.
    Authors: Peterson JE, Zurakowski D, Italiano JE
    Am. J. Hematol., 2010-07-01;85(7):487-93.
    Species: Human
    Sample Types: Cell Lysates
  23. Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment.
    Authors: Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C
    FASEB J., 2010-05-19;24(10):3782-95.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Role of vitamin D receptor in the antiproliferative effects of calcitriol in tumor-derived endothelial cells and tumor angiogenesis in vivo.
    Authors: Chung I, Han G, Seshadri M, Gillard BM, Yu WD, Foster BA, Trump DL, Johnson CS
    Cancer Res., 2009-01-13;69(3):967-75.
    Species: Mouse
    Sample Types: Cell Lysates
  25. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007-04-30;177(3):489-500.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human PDGF-BB DuoSet ELISA
By Tauseef Ahmad on 03/28/2018
Sample Tested: free pdgf,unencpasulated PDGF
Human PDGF-BB DuoSet ELISA DY220