Human PDGF-AA DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: PDGF-AA
The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).
Citations for Human PDGF-AA DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Mast Cell Tryptase Promotes Airway Remodeling by Inducing Anti-Apoptotic and Cell Growth Properties in Human Alveolar and Bronchial Epithelial Cells
Authors: Berlin, F;Mogren, S;Ly, C;Ramu, S;Hvidtfeldt, M;Uller, L;Porsbjerg, C;Andersson, CK;
Cells
Species: Human
Sample Types: Cell Culture Supernates
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Perioperative escape from dormancy of spontaneous micro-metastases: A role for malignant secretion of IL-6, IL-8, and VEGF, through adrenergic and prostaglandin signaling
Authors: R Haldar, LS Berger, E Rossenne, A Radin, A Eckerling, E Sandbank, EK Sloan, SW Cole, S Ben-Eliyah
Brain, Behavior, and Immunity, 2023-01-13;0(0):.
Species: Xenograft
Sample Types: Plasma
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Knock-out of 5-lipoxygenase in overexpressing tumor cells-consequences on gene expression and cellular function
Authors: H Weisser, T Göbel, G Melissa Kr, M Krei beta, C Angioni, D Sürün, D Thomas, T Schmid, AK Häfner, AS Kahnt
Cancer Gene Therapy, 2022-09-16;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Human Primary Astrocytes Differently Respond to Pro- and Anti-Inflammatory Stimuli
Authors: P Szpakowski, D Ksiazek-Wi, M Turniak-Ku, I Pacan, A Glabinski
Biomedicines, 2022-07-22;10(8):.
Species: Human
Sample Types: Cell Culture Supernates
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Systemic and mucosal immune profiling in asymptomatic and symptomatic SARS-CoV-2-infected individuals reveal unlinked immune signatures
Authors: S Ravichandr, G Grubbs, J Tang, Y Lee, C Huang, H Golding, S Khurana
Science Advances, 2021-10-13;7(42):eabi6533.
Species: Human
Sample Types: Nasal Lavage Fluid
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In Ovarian Cancer Multicellular Spheroids, Platelet Releasate Promotes Growth, Expansion of ALDH+ and CD133+ Cancer Stem Cells, and Protection against the Cytotoxic Effects of Cisplatin, Carboplatin and Paclitaxel
Authors: N Casagrande, C Borghese, F Agostini, C Durante, M Mazzucato, A Colombatti, D Aldinucci
International Journal of Molecular Sciences, 2021-03-16;22(6):.
Species: Human
Sample Types: Plasma
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Rescuing key native traits in cultured dermal papilla cells for human hair regeneration
Authors: CM Abreu, MT Cerqueira, RP Pirraco, L Gasperini, RL Reis, AP Marques
Journal of advanced research, 2020-11-04;30(0):103-112.
Species: Human
Sample Types: Cell Culture Supernates
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Oxidative stress-mediated mitochondrial dysfunction facilitates mesenchymal stem cell senescence in ankylosing spondylitis
Authors: G Ye, Z Xie, H Zeng, P Wang, J Li, G Zheng, S Wang, Q Cao, M Li, W Liu, S Cen, Z Li, Y Wu, Z Ye, H Shen
Cell Death Dis, 2020-09-17;11(9):775.
Species: Human
Sample Types: Serum
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Overexpression of Platelet-Derived Growth Factor and Its Receptor Are Correlated with Oral Tumorigenesis and Poor Prognosis in Oral Squamous Cell Carcinoma
Authors: LH Lin, JS Lin, CC Yang, HW Cheng, KW Chang, CJ Liu
Int J Mol Sci, 2020-03-29;21(7):.
Species: Human
Sample Types: Serum
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Induction of nuclear protein-1 by thyroid hormone enhances platelet-derived growth factor A mediated angiogenesis in liver cancer
Authors: CY Chen, SM Wu, YH Lin, HC Chi, SL Lin, CT Yeh, WY Chuang, KH Lin
Theranostics, 2019-04-13;9(8):2361-2379.
Species: Human
Sample Types: Cell Culture Supernates
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Measured plasma at 50-fold dilution, but samples were not in range. We did not have a chance to repeat at a lower dilution factor. Measured urine at 2-fold dilution and obtained values in range.
