Human PD-L1/B7-H1 PE-conjugated Antibody Summary
-H4, rhPD-L2, recombinant mouse B7-H1, recombinant rat (rr) B7-1, or rrB7-2 is observed.
Phe19-Thr239
Accession # Q9NZQ7
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of B7-H1/PD-L1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) untreated (A) or treated (B) overnight with 1 µg/mL LPS were stained with Mouse Anti-Human B7-H1/PD-L1 PE-conjugated Monoclonal Antibody (Catalog # FAB1561P) and Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
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Detection of PD-L1/B7-H1 in MDA-MB-231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast adenocarcinoma cell line was stained with Mouse Anti-Human PD-L1/B7-H1 PE-conjugated Monoclonal Antibody (Catalog # FAB1561P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). Adherent cells were prepared by either manual scraping or with TrypLE Express treatment with similar results. View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PD-L1/B7-H1
Human B7 homolog 1 (B7-H1), also called programmed death ligand 1 (PD-L1) and programmed cell death 1 ligand 1 (PDCD1L1), is a member of the growing B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. Other family members include B7-1, B7-2, B7-H2, PDL2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily. Their extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, there is about 20-25% amino acid identity. Human and mouse B7-H1 share approximately 70% amino acid sequence identity. B7-H1 has been identified as one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The B7-H1 gene encodes a 291 amino acid (aa) type I membrane precursor protein with a putative 18 aa signal peptide, a 220 aa extracellular domain, a 21 aa transmembrane region, and a 31 aa cytoplasmic domain. Human B7-H1 is constitutively expressed in several organs such as heart, skeletal muscle, placenta and lung, and in lower amounts in thymus, spleen, kidney and liver. B7-H1 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. B7-H1 expression is also induced in dendritic cells and keratinocytes after IFN-gamma stimulation. Interaction of B7-H1 with PD-1 results in inhibition of TCR-mediated proliferation and cytokine production. The B7-H1:PD-1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.
- Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265.
- Freeman, G.J. et al. (2000) J. Exp. Med. 192:1027.
- Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
Product Datasheets
Citations for Human PD-L1/B7-H1 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Prognostic significance of programmed cell death-ligand 1 expression on circulating tumor cells in various cancers: A systematic review and meta-analysis
Authors: Y Ouyang, W Liu, N Zhang, X Yang, J Li, S Long
Cancer Medicine, 2021-08-23;0(0):.
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Co-inhibition of adenosine 2b receptor and programmed death-ligand 1 promotes the recruitment and cytotoxicity of natural killer cells in oral squamous cell carcinoma
Authors: Wang, B;Wang, T;Yang, C;Nan, Z;Ai, D;Wang, X;Wang, H;Qu, X;Wei, F;
PeerJ
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Clinical potential of circulating free DNA and circulating tumour cells in patients with metastatic non‐small‐cell lung cancer treated with pembrolizumab
Authors: Patricia Mondelo‐Macía, Jorge García‐González, Luis León‐Mateos, Urbano Anido, Santiago Aguín, Ihab Abdulkader et al.
Molecular Oncology
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PD-L1 Expression Fluctuates Concurrently with Cyclin D in Glioblastoma Cells
Authors: M Tufano, P D'Arrigo, M D'Agostino, C Giordano, L Marrone, E Cesaro, MF Romano, S Romano
Cells, 2021-09-09;10(9):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Circulating Tumor Cell Detection and Polyomavirus Status in Merkel Cell Carcinoma
Authors: M Boyer, L Cayrefourc, F Garima, V Foulongne, O Dereure, C Alix-Panab
Sci Rep, 2020-01-31;10(1):1612.
Species: Human
Sample Types: Serum
Applications: ELISA Detection -
Monitoring PD-L1 positive circulating tumor cells in non-small cell lung cancer patients treated with the PD-1 inhibitor Nivolumab
Sci Rep, 2016-08-24;6(0):31726.
Species: Human
Sample Types: Whole Blood
Applications: Cell Selection -
Frequent expression of PD-L1 on circulating breast cancer cells.
Authors: Mazel M, Jacot W, Pantel K, Bartkowiak K, Topart D, Cayrefourcq L, Rossille D, Maudelonde T, Fest T, Alix-Panabieres C
Mol Oncol, 2015-06-09;9(9):1773-82.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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FEMX Human melanoma cells (1x10^6) were incubated for 30 minutes at 4C with 10 ul of Mouse B7-H1/PD-L1 PE-conjugated antibody (red), or PE-conjugated Mouse IgG1 (gray). Blocking: 1% BSA. Unfixed cells.
