Human PAUF/ZG16B Antibody

Catalog #: MAB7777
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7777
MAB7777-SP

Detection of Human PAUF/ZG16B by Western Blot.

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All PAUF/ZG16B Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PAUF/ZG16B Antibody Summary

Species Reactivity

Human

Specificity

Detects human PAUF/ZG16B in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG1 Clone # 817310

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PAUF/ZG16B by Western Blot. Western blot shows lysates of human placenta tissue and human prostate tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PAUF/ZG16B Monoclonal Antibody (Catalog # MAB7777) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PAUF/ZG16B at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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PAUF/ZG16B in Human Pancreas Cancer Tissue. PAUF/ZG16B was detected in immersion fixed paraffin-embedded sections of human pancreas cancer tissue using Mouse Anti-Human PAUF/ZG16B Monoclonal Antibody (Catalog # MAB7777) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membranes of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: PAUF/ZG16B

PAUF (pancreatic adenocarcinoma upregulated factor), gene name ZG16B (zymogen granule protein 16B), is a 156 amino acid, approximately 20-25 kDa secreted protein that is a member of the jacalin-binding lectin family. PAUF expression is thought to be restricted to primates, but the related ZG16P, which shares approximately 25% amino acid sequence identity, is more widely expressed. Human PAUF is overexpressed in pancreatic adenocarcinoma, binds Toll-like receptors TLR2 and TLR4, and inhibits CXCR4-dependent, TLR2-mediated NF-kB activation. It facilitates tumor growth, adhesiveness, and production of pro-tumorigenic cytokines.

Long Name

Pancreatic Adenocarcinoma Upregulated Factor

Entrez Gene IDs

124220 (Human)

Alternate Names

DKFZp762P2216; EC 3.1.2; EC 3.4.19.12; EECP; HRPE773; hTrabid; JCLN2; LOC124220; PAUF; PRO1567; TRABIDubiquitin thioesterase ZRANB1; TRAF-binding protein domain; ZG16B; Zinc finger Ran-binding domain-containing protein 1; zinc finger, RAN-binding domain containing 1

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human PAUF/ZG16B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1