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Human Pappalysin-1/PAPP-A ELISA Kit - Quantikine

Catalog #: DPPA00
5 Citations Datasheet / COA / SDS
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DPPA00
Control Products Available
QC23: Quantikine Immunoassay Control Group 8
Human Pappalysin-1/PAPP-A ELISA Standard Curve
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Product Details
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Citations (5)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Pappalysin-1/PAPP-A Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
5.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
0.116 ng/mL
Assay Range
0.8 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human PAPP-A
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human PAPP-A Immunoassay is a 5.5 hour sandwich-type solid phase ELISA designed to measure human PAPP-A in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human PAPP-A and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PAPP-A showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring PAPP-A.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 5.49 17.4 32.7 5.2 16.4 31.4
Standard Deviation 0.32 0.78 1.39 0.44 1.14 2.04
CV% 5.8 4.5 4.3 8.5 6.9 6.5

Recovery

The recovery of PAPP-A spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 110 90-117
EDTA Plasma (n=4) 97 89-108
Heparin Plasma (n=4) 95 86-111
Serum (n=4) 92 84-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PAPP-A were serially diluted in Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Pappalysin-1/PAPP-A ELISA Linearity

Scientific Data

N/A Human Pappalysin-1/PAPP-A ELISA Standard Curve View Larger

Human Pappalysin-1/PAPP-A ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Pappalysin-1/PAPP-A

Pappalysins comprise one of the five families of Metzincins (other families include ADAMs/ADAMTSs, MMPs, Astacins, and Serralysins). Pappalysin-1 (PAPP-A) is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy. Reduced maternal serum PAPP-A is a major marker of Down syndrome in the first trimester of pregnancy. PAPP-A cleaves IGFBP-4 and -5 (insulin-like growth factor-binding protein-4 and 5) at a single site, resulting in the release of bioactive IGF. Pappalysin-2 (PAPP-A2; also called PAPP-E) shares 45% amino acid identity with PAPP-A and cleaves IGFBP-2.

Long Name:
Pregnancy-associated Plasma Protein 1
Entrez Gene IDs:
5069 (Human); 18491 (Mouse)
Alternate Names:
ASBABP2; DIPLA1; EC 3.4.24.79; IGFBP-4ase; IGFBP-4aseaspecific BCL2 ARE-binding protein 2; IGF-dependent IGFBP-4 protease; insulin-like growth factor-dependent IGF binding protein-4 protease; Insulin-like growth factor-dependent IGF-binding protein 4 protease; PAPA; PAPPA; PAPPA1; PAPP-A1; PAPP-Adifferentially placenta 1 expressed protein; Pappalysin1; Pappalysin-1; pregnacy-associated plasma protein A; Pregnancy-associated plasma protein A; pregnancy-associated plasma protein A, pappalysin 1
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well within 15 minutes. Cover with a plate sealer, and incubate at room temperature for 3 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Pappalysin-1/PAPP-A Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Heavy Metals and Essential Metals Are Associated with Cerebrospinal Fluid Biomarkers of Alzheimer's Disease
    Authors: M Babi? Leko, M Mihel?i?, J Jurasovi?, M Nikolac Pe, E Špani?, A Sekovani?, T Orct, K Zub?i?, L Langer Hor, N Plei?, S Ki?emet-Pi, Ž Vogrinc, N Pivac, A Diana, F Borove?ki, PR Hof, G Šimi?
    International Journal of Molecular Sciences, 2022-12-27;24(1):.
    Species: Human
    Sample Types: CSF
  2. Parity predisposes breasts to the oncogenic action of PAPP-A and activation of the collagen receptor DDR2
    Authors: E Slocum, A Craig, A Villanueva, D Germain
    Breast Cancer Res., 2019-05-02;21(1):56.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Predicting first trimester pregnancy outcome: derivation of a multiple marker test
    Authors: S Senapati, MD Sammel, SF Butts, P Takacs, K Chung, KT Barnhart
    Fertil. Steril., 2016-10-26;106(7):1725-1732.e3.
    Species: Human
    Sample Types: Serum
  4. A proof-of-principle gel-free proteomics strategy for the identification of predictive biomarkers for the onset of pre-eclampsia.
    Authors: Blankley RT, Gaskell SJ, Whetton AD
    BJOG, 2009-08-04;116(11):1473-80.
    Species: Human
    Sample Types: Plasma
  5. Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients.
    Authors: Nishizawa H, Pryor-Koishi K, Suzuki M
    Mol. Hum. Reprod., 2008-09-18;14(10):595-602.
    Species: Human
    Sample Types: Serum

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