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Human Orexin R2/HCRTR2 Antibody

Catalog #: MAB5246 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5246
MAB5246-SP
Detection of Orexin R2/HCRTR2 in HEK293 Human Cell Line Transfected with Human Orexin R2/HCRTR2 and eGFP by Flow Cytometry.
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Human Orexin R2/HCRTR2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Orexin R2/HCRTR2. Stains human Orexin R2/HCRTR2 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2A Clone # 456738
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Orexin R2/HCRTR2
Met1-Trp444
Accession # AAC39602
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Orexin R2/HCRTR2 antibody in HEK293 Human Cell Line Transfected with Human Orexin R2/HCRTR2 and eGFP antibody by Flow Cytometry. View Larger

Detection of Orexin R2/HCRTR2 in HEK293 Human Cell Line Transfected with Human Orexin R2/HCRTR2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Orexin R2/HCRTR2 and eGFP was stained with either (A) Mouse Anti-Human Orexin R2/HCRTR2 Monoclonal Antibody (Catalog # MAB5246) or (B) Mouse IgG2AIsotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of Orexin R2/HCRTR2 antibody in A172 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Orexin R2/HCRTR2 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human Orexin R2/HCRTR2 Monoclonal Antibody (Catalog # MAB5246, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Orexin R2/HCRTR2

Hypocretin receptor 2 (HCRTR2); also known as orexin receptor 2 or OX2R is a 40 kDa 7-transmembrane G-protein-coupled glycoprotein that is a high affinity receptor for orexins A and B (hypocretins 1 and 2). In mouse brain, engagement of HCRTRs promotes wakefulness, such that absence of either orexins or their receptors creates a narcolepsy-like state. It also influences reward circuits involving food or addictive drugs. The extracellular portions of human HCRTR2 share 92% and 93% aa identity with corresponding portions of mouse and rat HCRTR2, respectively.

Long Name
Hypocretin Receptor Type 2
Entrez Gene IDs
3062 (Human); 387285 (Mouse); 25605 (Rat)
Alternate Names
HCRTR2; hypocretin (orexin) receptor 2; Hypocretin receptor type 2; Orexin R2; Orexin Receptor 2; orexin receptor type 2; OrexinR2; OX2R; Ox-2-R; ox2-R

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