Human Orexin R2/HCRTR2 Antibody

Catalog #: MAB52461 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB52461
MAB52461-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Orexin R2/HCRTR2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Orexin R2/HCRTR2. In flow cytometry, stains human Orexin R2/HCRTR2 transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2B Clone # 456723

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Orexin R2/HCRTR2
Met1-Trp444
Accession # AAC39602

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Orexin R2/HCRTR2 in Human Brain. Orexin R2/HCRTR2 was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using 25 µg/mL Mouse Anti-Human Orexin R2/HCRTR2 Monoclonal Antibody (Catalog # MAB52461) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Orexin R2/HCRTR2

Orexin R2 (Orexin Receptor 2), also known as HCRTR2 (Hypocretin Receptor 2) or OX2R is a 40 kDa 7-transmembrane G-protein-coupled glycoprotein that is a high affinity receptor for orexins A and B (hypocretins 1 and 2). In mouse brain, engagement of Orexin Rs/HCRTRs promotes wakefulness, such that absence of either orexins or their receptors creates a narcolepsy-like state. It also influences reward circuits involving food or addictive drugs. The extracellular portions of human Orexin R2/HCRTR2 share 92% and 93% aa identity with corresponding portions of mouse and rat Orexin R2/HCRTR2, respectively.

Long Name

Hypocretin Receptor Type 2

Entrez Gene IDs

3062 (Human); 387285 (Mouse); 25605 (Rat)

Alternate Names

HCRTR2; hypocretin (orexin) receptor 2; Hypocretin receptor type 2; Orexin R2; Orexin Receptor 2; orexin receptor type 2; OrexinR2; OX2R; Ox-2-R; ox2-R