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Human Oncostatin M (OSM) DuoSet ELISA

Catalog #: DY295
19 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY295
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human Oncostatin M / OSM ELISA Standard Curve
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Product Details
Procedure
Citations (19)
FAQs
Supplemental Products
Reviews (2)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human Oncostatin M (OSM) DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4 hours 40 minutes (after plate preparation)
Sample Volume Required
100 µL
Assay Range
31.2 - 2,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Oncostatin M (OSM). The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Human Oncostatin M / OSM ELISA Standard Curve View Larger

Human Oncostatin M / OSM ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Oncostatin M/OSM

Oncostatin M (OSM) is a cytokine originally isolated from medium conditioned by PMA-treated U-937 human histiocytic leukemia cells based on its ability to inhibit growth of A375 melanoma cells. The human OSM cDNA encodes a 252 amino acid pre-pro-OSM polypeptide with a 25 residue hydrophobic signal peptide and a hydrophilic C-terminal domain that are proteolytically processed to generate the 196 residue mature form of OSM. Although both mature and pro-OSM are equally active in radio-receptor assays, the mature OSM is 5- to 60-fold more active in growth inhibition assays. Thus, proteolytic processing of the pro-OSM peptide may be important in regulating the in vivo activities of OSM. OSM initiates its biological activities by binding to specific cell surface receptors. The gp130, a signal transducing component (beta subunit) of the IL-6, LIF and CNTF receptor complexes, was identified as a low-affinity OSM receptor that does not transduce OSM signals. The low affinity LIF receptor (LIF R, a gp130-related protein) has now been identified to be a component of a high-affinity OSM receptor that will transduce OSM signals. Since OSM is also active on cells that do not express LIF R, a specific OSM receptor that does not involve LIF R must also exist. Besides its growth inhibitory activities on human A375 melanoma and mouse M1 myeloid leukemic cells, as well as on other solid tumor cells, OSM also has growth stimulatory activities on normal fibroblasts, AIDS-Kaposis sarcoma cells, and a human erythroleukemia cell line, TF-1. Other OSM-mediated activities reported to date include: stimulation of plasminogen activator activity in cultured bovine aortic endothelial cells, regulation of IL-6 expression in human endothelial cells, and stimulation of LDL uptake and up-regulation of cell surface LDL receptors in HepG2 cells.

Human Oncostatin M (OSM) signals through two types of human OSM receptor complexes: the type I complex comprising the leukemia inhibitory factor receptor beta (LIF R beta) and gp130 and the type II complex made up of OSM receptor beta (OSM R beta) and gp130. In contrast, mouse OSM signals only through the mouse OSM R beta and gp130 complex. Human and mouse OSM R beta are 55% identical at the amino acid sequence level.

Entrez Gene IDs:
5008 (Human); 18413 (Mouse)
Alternate Names:
MGC20461; oncostatin M; oncostatin-M; OSM

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

 

Citations for Human Oncostatin M (OSM) DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Nuclear factor-?B activation by transforming growth factor-?1 drives tumour microenvironment-mediated drug resistance in neuroblastoma
    Authors: Louault, K;Blavier, L;Lee, MH;Kennedy, RJ;Fernandez, GE;Pawel, BR;Asgharzadeh, S;DeClerck, YA;
    British journal of cancer
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Heterogeneity in the Metastatic Microenvironment: JunB-Expressing Microglia Cells as Potential Drivers of Melanoma Brain Metastasis Progression
    Authors: Adir, O;Sagi-Assif, O;Meshel, T;Ben-Menachem, S;Pasmanik-Chor, M;Hoon, DSB;Witz, IP;Izraely, S;
    Cancers
    Species: Human
    Sample Types: Cell Culture Supernates, Cell Lysates
  3. Mast cells disrupt the function of the esophageal epithelial barrier
    Authors: Kleuskens, MTA;Bek, MK;Al Halabi, Y;Blokhuis, BRJ;Diks, MAP;Haasnoot, ML;Garssen, J;Bredenoord, AJ;C A M van Esch, B;Redegeld, FA;
    Mucosal immunology
    Species:  Human
    Sample Types: Cell Culture Supernates
  4. Oncostatin M and its receptor in women with polycystic ovary syndrome and association with assisted reproductive technology outcomes
    Authors: S Nikanfar, K Hamdi, S Haiaty, N Samadi, V Shahnazi, A Fattahi, M Nouri
    Reproductive biology, 2022-03-09;22(2):100633.
    Species: Human
    Sample Types: Follicular Fluid
  5. Treponema denticola stimulates Oncostatin M cytokine release and de novo synthesis in neutrophils and macrophages
    Authors: MM Jones, ST Vanyo, W Ibraheem, A Maddi, MB Visser
    J. Leukoc. Biol., 2020-07-17;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  6. miRNA551b-3p Activates an Oncostatin Signaling Module for the Progression of Triple-Negative Breast Cancer
    Authors: D Parashar, A Geethadevi, MR Aure, J Mishra, J George, C Chen, MK Mishra, A Tahiri, W Zhao, B Nair, Y Lu, LS Mangala, C Rodriguez-, G Lopez-Bere, AKS Camara, M Liang, JS Rader, R Ramchandra, M You, AK Sood, VN Kristensen, GB Mills, S Pradeep, P Chaluvally
    Cell Rep, 2019-12-24;29(13):4389-4406.e10.
    Species: Mouse
    Sample Types: Serum
  7. OSM potentiates preintravasation events, increases CTC counts, and promotes breast cancer metastasis to the lung
    Authors: K Tawara, C Bolin, J Koncinsky, S Kadaba, H Covert, C Sutherland, L Bond, J Kronz, JR Garbow, CL Jorcyk
    Breast Cancer Res., 2018-06-14;20(1):53.
    Species: Xenograft
    Sample Types: Serum
  8. JAK1/STAT3 activation through a proinflammatory cytokine pathway leads to resistance to molecularly targeted therapy in non-small cell lung cancer
    Authors: K Shien, VA Papadimitr, D Ruder, C Behrens, L Shen, N Kalhor, J Song, JJ Lee, J Wang, X Tang, RS Herbst, S Toyooka, L Girard, JD Minna, JM Kurie, II Wistuba, JG Izzo
    Mol. Cancer Ther., 2017-07-20;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Overexpression of the oncostatin-M receptor in cervical squamous cell carcinoma is associated with epithelial-mesenchymal transition and poor overall survival
    Authors: JA Kucia-Tran, V Tulkki, S Smith, CG Scarpini, K Hughes, AM Araujo, KY Yan, J Botthof, E Pérez-Góme, M Quintanill, K Cuschieri, MM Caffarel, N Coleman
    Br. J. Cancer, 2016-06-28;115(2):212-22.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Osteopontin Promotes Oncostatin M Production in Human Osteoblasts: Implication of Rheumatoid Arthritis Therapy.
    Authors: Su C, Chiang Y, Huang C, Hsu C, Fong Y, Tang C
    J Immunol, 2015-08-24;195(7):3355-64.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. CCN1 induces oncostatin M production in osteoblasts via integrin-dependent signal pathways.
    Authors: Chen C, Su C, Huang Y, Tsai C, Fuh L, Tang C
    PLoS ONE, 2014-09-04;9(9):e106632.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation.
    Authors: Nicolaidou V, Wong MM, Redpath AN
    PLoS ONE, 2012-07-03;7(7):e39871.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Induction of CCL13 expression in synovial fibroblasts highlights a significant role of oncostatin M in rheumatoid arthritis.
    Authors: Hintzen C, Quaiser S, Pap T, Heinrich PC, Hermanns HM
    Arthritis Rheum., 2009-07-01;60(7):1932-43.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
    Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
    Blood, 2007-09-11;110(13):4319-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Oncostatin M enhances CCL21 expression by microvascular endothelial cells and increases the efficiency of dendritic cell trafficking to lymph nodes.
    Authors: Sugaya M, Fang L, Cardones AR, Kakinuma T, Jaber SH, Blauvelt A, Hwang ST
    J. Immunol., 2006-12-01;177(11):7665-72.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Breast cancer cells stimulate neutrophils to produce oncostatin M: potential implications for tumor progression.
    Authors: Queen MM, Ryan RE, Holzer RG, Keller-Peck CR, Jorcyk CL
    Cancer Res., 2005-10-01;65(19):8896-904.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Effect of azacytidine in the release of leukemia inhibitory factor, oncostatin m, interleukin (IL)-6, and IL-11 by mononuclear cells of patients with refractory anemia.
    Authors: Lopez-Karpovitch X, Barrales-Benitez O, Flores M, Piedras J
    Cytokine, 2002-11-24;20(4):154-62.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Coexpression of oncostatin M and its receptors and evidence for STAT3 activation in human ovarian carcinomas.
    Authors: Savarese TM, Campbell CL, McQuain C, Mitchell K, Guardiani R, Quesenberry PJ, Nelson BE
    Cytokine, 2002-03-21;17(6):324-34.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Oncostatin M production by human dendritic cells in response to bacterial products.
    Authors: Suda T, 146, Chida K, Todate A, Ide K, Asada K, Nakamura Y, Suzuki K, Kuwata H, Nakamura H
    2002-03-21;17(6):335-40.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human Oncostatin M (OSM) DuoSet ELISA
By Anonymous on 09/18/2018
Sample Tested: Serum
Human Oncostatin M (OSM) DuoSet ELISA
By Anonymous on 09/30/2016
Sample Tested: Skeletal muscle