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Human OCTN2/SLC22A5 Antibody

Catalog #: MAB11004 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB11004-100
MAB11004-SP
Detection of OCTN2/SLC22A5 in HEK293 Human Cell Line Transfected with Human OCTN2/SLC22A5 and eGFP by Flow Cytometry
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Human OCTN2/SLC22A5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human OCTN2/SLC22A5 in ELISA.
Source
Monoclonal Mouse IgG2A Clone # 893753
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 transfected with human SLC22A5
Accession # O76082
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
HEK293 Human Cell Line Transfected with Human OCTN2/SLC22A5 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of OCTN2/SLC22A5 in HEK293 Human Cell Line Transfected with Human OCTN2/SLC22A5 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with (A) human OCTN2/SLC22A5 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human OCTN2/SLC22A5 Monoclonal Antibody (Catalog # MAB11004) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OCTN2/SLC22A5

Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for the elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. SLC22A5 is an integral plasma membrane protein which functions as an organic cation transporter and as a sodium-dependent high affinity carnitine transporter. Mutations in SLC22A5 are the cause of systemic primary carnitine deficiency (CDSP), an autosomal recessive disorder manifested early in life by hypoketotic hypoglycemia and acute metabolic decompensation, and later in life by skeletal myopathy or cardiomyopathy.

Long Name
Solute Carrier Family 22 (Organic Cation/Carnitin Transporter) Member 5
Entrez Gene IDs
6584 (Human); 20520 (Mouse); 29726 (Rat)
Alternate Names
CDSP; FLJ46769; High-affinity sodium-dependent carnitine cotransporter; OCTN2; OCTN2high-affinity sodium dependent carnitine cotransporter; OCTN2VT; organic cation transporter 2; organic cation transporter 5; Organic cation/carnitine transporter 2; SCD; SLC22A5; solute carrier family 22 (organic cation transporter), member 5; solute carrier family 22 (organic cation/carnitine transporter), member 5; solute carrier family 22 member 5

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