Human OCTN1/SLC22A4 Antibody

Catalog #: MAB10985 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10985-100
MAB10985-SP

Detection of OCTN1/SLC22A4 in HEK293 Human Cell Line Transfected with Human OCTN1/SLC22A4 and eGFP by Flow Cytometry.

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Human OCTN1/SLC22A4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human OCTN1/SLC22A4 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 892147

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human SLC22A4
Accession # Q9H015

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 Human Cell Line Transfected with Human OCTN1/SLC22A4 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of OCTN1/SLC22A4 in HEK293 Human Cell Line Transfected with Human OCTN1/SLC22A4 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human OCTN1/SLC22A4 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human OCTN1/SLC22A4 Monoclonal Antibody (Catalog # MAB10985) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OCTN1/SLC22A4

Polyspecific organic cation transporters are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. SLC22A4 is an organic cation transporter and integral plasma membrane protein containing eleven putative transmembrane domains as well as a nucleotide-binding site motif. Transport by SLC22A4 is at least partially ATP-dependent. SLC22A4 may play a role in Crohn's disease.

Long Name

Solute Carrier Family 22 (Organic Cation/Ergothioneine Transporter) Member 4

Entrez Gene IDs

6583 (Human); 30805 (Mouse)

Alternate Names

Ergothioneine transporter; ET transporter; ETT; MGC34546; MGC40524; OCTN1; OCTN1integral membrane transport protein; Organic cation/carnitine transporter 1; SLC22A4; solute carrier family 22 (organic cation/ergothioneine transporter), member 4; solute carrier family 22 member 4; UT2H

Product Datasheets

Product Datasheet

COA

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