Human NTB-A/SLAMF6 Fluorescein-conjugated Antibody

Catalog #: FAB19081F Datasheet / COA / SDS

Clone 292811 was used by HLDA to establish CD designation

Catalog #	Availability	Size / Price	Qty
FAB19081F-025
FAB19081F-100

Detection of NTB‑A/SLAMF6 in Human Blood Lymphocytes by Flow Cytometry.

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Human NTB-A/SLAMF6 Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human NTB‑A/SLAMF6 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) BLAME or rhCRACC is observed.

Source

Monoclonal Mouse IgG_2A Clone # 292811

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NTB‑A/SLAMF6
Leu28-Lys225
Accession # Q96DU3

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of NTB‑A/SLAMF6 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human NTB-A/SLAMF6 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB19081F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: NTB-A/SLAMF6

NTB-A (NK-T-B-antigen), also known as Ly108 and SLAMF6, is a 60 kDa type I transmembrane glycoprotein that belongs to the SLAM subgroup of the CD2 family (1). Mature human NTB-A contains a 205 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set and one Ig-like C2-set domain. It also contains a 21 aa transmembrane segment and an 84 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs (ITSMs) (2, 3). An alternately spliced isoform is truncated in the cytoplasmic domain and lacks the two ITSMs. Within the ECD, human NTB-A shares 48% aa sequence identity with mouse and rat NTB-A. The ECD of human NTB-A shares 19%‑34% aa sequence identity with comparable regions of human 2B4, BLAME, CD2F-10, CD84, CD229, CRACC, and SLAM. NTB-A is expressed on the surface of NK, T, and B lymphocytes as well as eosinophils (2, 4, 5). It interacts homophilically through weak associations between the Ig-V domains (2, 5-7). NTB-A functions as an activating coreceptor on NK and T cells (2, 5, 6, 8). Tyrosine phosphorylation in the membrane proximal ITSM enables specific association with EAT-2, an interaction that is required for NTB-A mediated cytotoxicity of NK cells (9). Phosphorylation-dependent NTB-A association with SAP is required for full production of IFN-gamma by NK cells (5, 9). This interaction is independent of EAT-2 binding and appears to involve the membrane distal ITSM (5, 9). NTB-A deficient mice show weakened Th2 responses and elevated levels of neutrophil-derived inflammatory mediators (10). On B cells, NTB-A modulates immunoglobulin class switching and the balance between tolerance and autoimmunity (5, 11).

References

Veillette, A. (2006) Immunol. Rev. 214:22.
Bottino, C. et al. (2001) J. Exp. Med. 194:235.
Fraser, C.C. et al. (2002) Immunogenetics 53:843.
Munitz, A. et al. (2005) J. Immunol. 174:110.
Valdez, P.A. et al. (2004) J. Biol. Chem. 279:18662.
Flaig, R.M. et al. (2004) J. Immunol. 172:6524.
Cao, E. et al. (2006) Immunity 25:559.
Stark, S. and C. Watzl (2006) Int. Immunol. 18:241.
Eissmann, P. and C. Watzl (2006) J. Immunol. 177:3170.
Howie, D. et al. (2005) J. Immunol. 174:5931.
Kumar, K.R. et al. (2006) Science 312:1665.

Long Name

NK-T-B Antigen/SLAM Family Member 6

Entrez Gene IDs

114836 (Human)

Alternate Names

Activating NK receptor; CD352 antigen; CD352; KALIFLJ50657; Ly108; NK-T-B-antigen; NTBA receptor; NTBA; NTB-A; NTB-AMGC104953; NTBAT- and B-cell antigen; SF2000; SLAM family member 6; SLAMF6