Human NT-ProANP Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.71 | 6.04 | 11.1 | 1.81 | 5.95 | 11.5 |
| Standard Deviation | 0.069 | 0.224 | 0.583 | 0.129 | 0.426 | 0.842 |
| CV% | 4 | 3.7 | 5.3 | 7.1 | 7.2 | 7.3 |
Recovery
The recovery of human NT-ProANP spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 104 | 87-114 |
| EDTA Plasma (n=4) | 102 | 84-120 |
| Heparin Plasma (n=4) | 98 | 84-111 |
| Serum (n=4) | 108 | 95-122 |
Linearity
Scientific Data
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Endothelin Induces Expression of Atrial Natriuretic Peptide in iPSC-derived Cardiomyoctyes. JOY6 human iPSCs were differentiated into beating cardiomyocytes. After 21 days of differentiation cardiomyocytes were treated with 5 nM of Endothelin-1 (Catalog # 1160) for 25.5 hours to induce cardiac hypertrophy. The level of secreted Pro-Atrial Natriuretic peptide (ANP), which is known to be elevated during cardiac hypertrophy, was assessed using the Human NT-ProANP Quantikine® ELISA Kit (Catalog # DANP00). As expected, detection of Pro-ANP was increased in Endothelin-1 treated cells.
Product Datasheets
Preparation and Storage
Background: Atrial Natriuretic Peptide/ANP
Atrial Natriuretic peptide (ANP) is synthesized as a 151 aa preproprotein. The proprotein contains cardiodilatin (aa 26 - 92) and atrial natriuretic factor (ANF; aa 124 - 151) which become active after processing by the serine protease corin. ANP is primarily synthesized in the heart and is secreted in response to atrial distention. ANF circulates in the plasma, eliciting natriuretic, diuretic, vasorelaxant and antimitogenic effects directed toward reduction of body fluid and blood pressure regulation. ANF acts through the cGMP-coupled NP receptor A. Human ANP and ANF share 84% and 96% aa identity with mouse ANP and ANF, respectively.
Citations for Human NT-ProANP Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Dapagliflozin Improved Cardiac Function and Structure in Diabetic Patients with Preserved Ejection Fraction: Results of a Single Centre, Observational Prospective Study
Authors: Cortés, M;Lorenzo, O;Lumpuy-Castillo, J;Martínez-Albaladejo, S;Taibo-Urquía, M;Pello, AM;Bollas, AJ;Orejas, M;Navas, MÁ;Macia, E;Martínez, ME;Rueda, A;Tuñón, J;
Journal of clinical medicine
Species: Human
Sample Types: Plasma, Serum, Urine
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Associations of atrial natriuretic peptide with measures of insulin and adipose depots
Authors: CA Couch, LA Fowler, V Parcha, P Arora, BA Gower
Physiological Reports, 2023-03-01;11(5):e15625.
Species: Human
Sample Types: Serum
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Associations among NPPA gene polymorphisms, serum ANP levels, and hypertension in the Chinese Han population
Authors: H Zhang, X Mo, Z Zhou, Z Zhu, X HuangFu, T Xu, A Wang, Z Guo, Y Zhang
J Hum Hypertens, 2019-07-24;0(0):.
Species: Human
Sample Types: Serum
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