Human NMNAT-1 Antibody

Catalog #: AF5865 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5865
AF5865-SP

Detection of Human NMNAT‑1 by Western Blot.

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Human NMNAT-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NMNAT‑1 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human NMNAT-1 (Catalog # 5865‑NT), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NMNAT‑1 by Western Blot. Western blot shows lysates of human muscle tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human NMNAT-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5865) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NMNAT-1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: NMNAT-1

NMNAT-1 is expressed in the nuclei of all human tissues, with highest expression in skeletal muscle, heart, kidney, pancreas, and brain (1). The enzyme transfers adenylate from ATP to nicotinamide ribonucleotide or nicotinate ribonucleotide to generate NAD⁺ or deamido-NAD⁺, and is an essential enzyme for the production of nuclear NAD⁺ (2). Nuclear NAD⁺ is required by poly(ADP-ribose) polymerase 1 (PARP-1), which poly-ADP-ribosylates chromatin in response to DNA strand breaks. NMNAT-1 is known to interact with PARP-1, resulting in its activation, but this interaction with PARP-1 is prevented when NMNAT-1 is phosphorylated at Ser136 (3). Nuclear NAD⁺ levels are also important for the regulation of SIR2 histone deacetylases (4). A naturally occurring Ube4b/NMNAT-1 chimeric protein is directly involved in slowing the degeneration of injured neurons in mice (5). NMNAT activity is required for the activation of tiazofurin, a drug used to treat leukemia (6). Two other NMNAT enzymes are present in humans. NMNAT-2 is localized in the Golgi complex and cytoplasm, and NMNAT-3 is a mitochondrial enzyme (7).

References

Emanuelli, M. et al. (2001) J. Biol.Chem. 276:406.
Schweiger, M. et al. (2001) FEBS Lett. 492:95.
Berger, F. et al. (2007) Proc. Natl. Acad. Sci. USA 104:3765.
Revollo, J.R. et al. (2004) J. Biol. Chem. 279:50754.
Mack, T.G. et al. (2001) Nature Neurosci. 4:1199.
Boulton, S. et al. (1997) Br. J. Cancer 76:845.
Berger, F. et al. (2005) J. Biol. Chem. 280:36334.

Long Name

Nicotinamide mononucleotide adenylyltransferase-1

Entrez Gene IDs

64802 (Human)

Alternate Names

EC 2.7.7.1; EC 2.7.7.18; NaMN adenylyltransferase 1; nicotinamide mononucleotide adenylyltransferase 1; nicotinamide nucleotide adenylyltransferase 1; nicotinamide nucleotide adenylyltransferase; Nicotinate-nucleotide adenylyltransferase 1; NMN adenylyltransferase 1; NMNAT1; NMNAT-1; NMNATPNAT1; PNAT1; pyridine nucleotide adenylyltransferase 1