Home / NKG2D/CD314 / Human NKG2D/CD314 PE-conjugated Antibody

Human NKG2D/CD314 PE-conjugated Antibody

Catalog #: FAB139P
33 Citations Datasheet / COA / SDS
Clone 149810 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB139P-025
FAB139P-100
Detection of NKG2D/CD314 in Human PBMCs gated on CD3-cells by Flow Cytometry.
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Citations (33)
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Human NKG2D/CD314 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human NKG2D/CD314 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 149810
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human NKG2D/CD314
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of NKG2D/CD314 antibody in Human PBMCs gated on CD3<sup>-</sup>cells antibody by Flow Cytometry. View Larger

Detection of NKG2D/CD314 in Human PBMCs gated on CD3-cells by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD3-cells were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2D/CD314 PE-conjugated Monoclonal Antibody (Catalog # FAB139P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: NKG2D/CD314

NKG2D, also known as CD314, is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells, and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

References
  1. Li, P. et al. (2001) Nature Immunol. 2:443.
  2. Steinle, A. et al. (2001) Immunogenetics 53:279.
  3. Cosman, D. et al. (2001) Immunity 14:123.
  4. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
  5. Wu, J. et al. (1999) Science 285:730.
  6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
  7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
  8. Groh, V. et al. (2001) Nature Immunol. 2:255.
  9. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
  10. Diefenbach, A. et al. (2001) Nature 413:165.
  11. NKG2D and its Ligands (2002) www.RnDSystems.com.
Long Name
Natural Killer G2D
Entrez Gene IDs
22914 (Human); 27007 (Mouse); 102120479 (Cynomolgus Monkey)
Alternate Names
CD314 antigen; CD314; D12S2489E; FLJ17759; FLJ75772; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; KLRK1; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2D; NKG2-D; NKG2-D-activating NK receptor; NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

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Citations for Human NKG2D/CD314 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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  1. Elevated Levels of Circulating Hsp70 and an Increased Prevalence of CD94+/CD69+ NK Cells Is Predictive for Advanced Stage Non-Small Cell Lung Cancer
    Authors: Sophie Seier, Ali Bashiri Dezfouli, Philipp Lennartz, Alan Graham Pockley, Henriette Klein, Gabriele Multhoff
    Cancers (Basel)
  2. Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement
    Authors: R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti
    Cell Reports, 2022-09-27;40(13):111423.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Potential Role of Hsp70 and Activated NK Cells for Prediction of Prognosis in Glioblastoma Patients
    Authors: Dominik Lobinger, Jens Gempt, Wolfgang Sievert, Melanie Barz, Sven Schmitt, Huyen Thie Nguyen et al.
    Frontiers in Molecular Biosciences
  4. Functionally Relevant Differences in Plasma Fatty Acid Composition and Expression of Cytotoxic and Inhibitory NK Cell Receptors between Healthy Young and Healthy Elder Adults
    Authors: JB De Sanctis, DC Dumut, D Radzioch, M Hajdúch
    Nutrients, 2020-11-26;12(12):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Expressions of natural cytotoxicity receptor, NKG2D and NKG2D ligands in endometriosis
    Authors: H Xu
    J. Reprod. Immunol., 2019-09-28;136(0):102615.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. EGFR-Specific Tyrosine Kinase Inhibitor Modifies NK Cell-Mediated Antitumoral Activity against Ovarian Cancer Cells
    Authors: N Mallmann-G, Y Sax, R Kimmig, S Lang, S Brandau
    Int J Mol Sci, 2019-09-22;20(19):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Cryopreserved Human Natural Killer Cells Exhibit Potent Antitumor Efficacy against Orthotopic Pancreatic Cancer through Efficient Tumor-Homing and Cytolytic Ability (Running Title: Cryopreserved NK Cells Exhibit Antitumor Effect)
    Authors: E Oh, B Min, Y Li, C Lian, J Hong, GM Park, B Yang, SY Cho, YK Hwang, CO Yun
    Cancers (Basel), 2019-07-09;11(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Histone Deacetylase Inhibitor Modulates NKG2D Receptor Expression and Memory Phenotype of Human Gamma/Delta T Cells Upon Interaction With Tumor Cells
    Authors: Jaydeep Bhat, Samuel Dubin, Alexandra Dananberg, Elgar Susanne Quabius, Juergen Fritsch, C. Marie Dowds et al.
    Frontiers in Immunology
  9. Ex vivo-expanded highly purified natural killer cells in combination with temozolomide induce antitumor effects in human glioblastoma cells in vitro
    Authors: Y Tanaka, T Nakazawa, M Nakamura, F Nishimura, R Matsuda, K Omoto, Y Shida, T Murakami, I Nakagawa, Y Motoyama, H Morita, T Tsujimura, H Nakase
    PLoS ONE, 2019-03-06;14(3):e0212455.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Large scale ex vivo expansion of clinical‑grade effector cells for adoptive immunotherapy
    Authors: Huiying Yu, Wei Chen, Changling Li, Di Lin, Junde Liu, Zien Yang et al.
    Experimental and Therapeutic Medicine
  11. Activated hepatic stellate cells impair NK cell anti-fibrosis capacity through a TGF-?-dependent emperipolesis in HBV cirrhotic patients
    Authors: J Shi, J Zhao, X Zhang, Y Cheng, J Hu, Y Li, X Zhao, Q Shang, Y Sun, B Tu, L Shi, B Gao, FS Wang, Z Zhang
    Sci Rep, 2017-03-14;7(0):44544.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Interferon Alpha Induces Sustained Changes in NK Cell Responsiveness to Hepatitis B Viral Load Suppression In Vivo
    PLoS Pathog, 2016-08-03;12(8):e1005788.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Natural killer (NK) cells inhibit systemic metastasis of glioblastoma cells and have therapeutic effects against glioblastomas in the brain.
    Authors: Lee S, Kang W, Yoon Y, Jin J, Song H, Her J, Kang S, Hwang Y, Kang K, Joo K, Nam D
    BMC Cancer, 2015-12-24;15(1):1011.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Up-regulation of activating and inhibitory NKG2 receptors in allogeneic and autologous hematopoietic stem cell grafts.
    Authors: Picardi A, Mengarelli A, Marino M, Gallo E, Benevolo M, Pescarmona E, Cocco R, Fraioli R, Tremante E, Petti M, De Fabritiis P, Giacomini P
    J Exp Clin Cancer Res, 2015-09-11;34(0):98.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure.
    Authors: Zou Y, Bao J, Pan X, Lu Y, Liao S, Wang X, Wang G, Lin D
    PLoS ONE, 2015-08-04;10(8):e0134568.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Human Induced Pluripotent Stem Cells Are Targets for Allogeneic and Autologous Natural Killer (NK) Cells and Killing Is Partly Mediated by the Activating NK Receptor DNAM-1.
    Authors: Kruse V, Hamann C, Monecke S, Cyganek L, Elsner L, Hubscher D, Walter L, Streckfuss-Bomeke K, Guan K, Dressel R
    PLoS ONE, 2015-05-07;10(5):e0125544.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. NZ28-induced inhibition of HSF1, SP1 and NF-kappa B triggers the loss of the natural killer cell-activating ligands MICA/B on human tumor cells
    Authors: Daniela Schilling, Annett Kühnel, Fabian Tetzlaff, Sarah Konrad, Gabriele Multhoff
    Cancer Immunology, Immunotherapy
  18. NKG2D-directed cytokine-activated killer lymphocyte therapy combined with gemcitabine for patients with chemoresistant metastatic solid tumors.
    Authors: Morisaki T, Hirano T, Koya N, Kiyota A, Tanaka H, Umebayashi M, Onishi H, Katano M
    Anticancer Res, 2014-08-01;34(8):4529-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. An epithelial cell adhesion molecule- and CD3-bispecific antibody plus activated T-cells can eradicate chemoresistant cancer stem-like pancreatic carcinoma cells in vitro.
    Authors: Umebayashi M, Kiyota A, Koya N, Tanaka H, Onishi H, Katano M, Morisaki T
    Anticancer Res, 2014-08-01;34(8):4509-19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. c-Cbl regulates MICA- but not ULBP2-induced NKG2D down-modulation in human NK cells.
    Authors: Molfetta R, Quatrini L, Capuano C, Gasparrini F, Zitti B, Zingoni A, Galandrini R, Santoni A, Paolini R
    Eur J Immunol, 2014-06-17;44(9):2761-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Long-term effect on natural killer cells by interferon-alpha therapy on the outcomes of HCV infection.
    Authors: Hu X, Jiang Y, Li X, Gao Y, Guo X, Chi X, Yan H, Feng J, Zhong J, Sun B, Shao X, Xiao W, Pan Y, Niu J
    J Interferon Cytokine Res, 2013-12-24;34(5):366-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Natural killer cells are characterized by the concomitantly increased interferon-gamma and cytotoxicity in acute resolved hepatitis B patients.
    Authors: Zhao J, Li Y, Jin L, Zhang S, Fan R, Sun Y, Zhou C, Shang Q, Li W, Zhang Z, Wang F
    PLoS ONE, 2012-11-01;7(11):e49135.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Combinatorial Cytotoxicity of Gemcitabine and Cytokine-activated Killer Cells in Hepatocellular Carcinoma via the NKG2D-MICA/B System.
    Authors: Morisaki T, Onishi H, Koya N
    Anticancer Res., 2011-07-01;31(7):2505-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Natural killer cells from children with type 1 diabetes have defects in NKG2D-dependent function and signaling.
    Authors: Qin H, Lee IF, Panagiotopoulos C, Wang X, Chu AD, Utz PJ, Priatel JJ, Tan R
    Diabetes, 2011-01-26;60(3):857-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry.
    Authors: Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P
    Immunol. Lett., 2010-10-15;134(2):113-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Phenotype markers and cytokine intracellular production by CD8+ gammadelta T lymphocytes do not support a regulatory T profile in Behcet's disease patients and healthy controls.
    Authors: Clemente A, Cambra A, Munoz-Saa I, Crespi C, Pallares L, Juan A, Matamoros N, Julia MR
    Immunol. Lett., 2010-02-16;129(2):57-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC.
    Authors: Inagaki A, Ishida T, Yano H, Ishii T, Kusumoto S, Ito A, Ri M, Mori F, Ding J, Komatsu H, Iida S, Ueda R
    Int. J. Cancer, 2009-07-01;125(1):212-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. Binding of excreted and/or secreted products of adult hookworms to human NK cells in Necator americanus-infected individuals from Brazil.
    Authors: Teixeira-Carvalho A, Fujiwara RT, Stemmy EJ, Olive D, Damsker JM, Loukas A, Correa-Oliveira R, Constant SL, Bethony JM
    Infect. Immun., 2008-10-06;76(12):5810-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  29. Functional impairment in circulating and intrahepatic NK cells and relative mechanism in hepatocellular carcinoma patients.
    Authors: Cai L, Zhang Z, Zhou L, Wang H, Fu J, Zhang S, Shi M, Zhang H, Yang Y, Wu H, Tien P, Wang FS
    Clin. Immunol., 2008-09-27;129(3):428-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity.
    Authors: Kwong KY, Baskar S, Zhang H, Mackall CL, Rader C
    J. Mol. Biol., 2008-09-16;384(5):1143-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. Endometrial NK cells are special immature cells that await pregnancy.
    Authors: Manaster I, Mizrahi S, Goldman-Wohl D, Sela HY, Stern-Ginossar N, Lankry D, Gruda R, Hurwitz A, Bdolah Y, Haimov-Kochman R, Yagel S, Mandelboim O
    J. Immunol., 2008-08-01;181(3):1869-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. 4-1BB regulates NKG2D costimulation in human cord blood CD8+ T cells.
    Authors: Kim YJ, Han MK, Broxmeyer HE
    Blood, 2007-11-16;111(3):1378-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  33. Phosphostim-activated gamma delta T cells kill autologous metastatic renal cell carcinoma.
    Authors: Viey E, Fromont G, Escudier B, Morel Y, Da Rocha S, Chouaib S, Caignard A
    J. Immunol., 2005-02-01;174(3):1338-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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