Human NF-H Antibody

Catalog # Availability Size / Price Qty
MAB31082-100
MAB31082-SP
Detection of Human NF‑H by Western Blot.
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Human NF-H Antibody Summary

Species Reactivity
Human
Specificity
Detects human NF-H in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 1016103
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human NF-H peptide
Accession # P12036
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
IMR‑32 human neuroblastoma cell line
Simple Western
5 µg/mL
Human motor cortex and human hippocampus

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human NF‑H by Western Blot. Western blot shows lysates of IMR‑32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NF‑H Monoclonal Antibody (Catalog # MAB31082) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for NF‑H at approximately 210 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western View Larger

Detection of Human NF‑H by Simple WesternTM. Simple Western shows lysates of human motor cortex and human hippocampus, loaded at 0.2 mg/ml. A specific band was detected for NF‑H at approximately 270-350 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human NF‑H Monoclonal Antibody (Catalog # MAB31082). This experiment was conducted under reducing conditions and using the 66-440kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NF-H

The human Neurofilament Heavy chain, also known as NF-H and NEFH, is a phosphorylated cytoskeletal intermediate filament protein that is expressed in neurons. Neurofilaments are trimers that always contain the 68 kDa NF-L and variably contain 125 kDa NF-M and 200 kDa NF-H. In the region used for immunization, mouse and rat NF-H are each 93% identical to human NF-H.

Long Name
Neurofilament Protein, Heavy Chain
Entrez Gene IDs
4744 (Human)
Alternate Names
200 kDa neurofilament protein; KIAA0845; NEFH; Neurofilament Heavy (200kDa); neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; neurofilament, heavy polypeptide; NF200; NFH; NF-H

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