Human NCAM-1/CD56 Antibody

Catalog #: MAB24083 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB24083-100
MAB24083-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NCAM-1/CD56 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NCAM-1/CD56 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1006435

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NCAM-1/CD56
Leu20-Pro603 and Glu636-Asn741
Accession # NP_001070150

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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NCAM‑1/CD56 in NK‑92 Human Cell Line. NCAM-1/CD56 was detected in immersion fixed NK-92 human natural killer lymphoma cell line (positive staining) and MCF-7 human breast cancer cell line (negative staining) using Mouse Anti-Human NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB24083) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NCAM-1/CD56

Neural cell adhesion molecule 1 (NCAM-1) is a multifunctional member of the Ig superfamily. It belongs to a family of membrane-bound glycoproteins that are involved in Ca⁺⁺ independent cell matrix and homophilic or heterophilic cell-cell interactions. NCAM-1 specifically binds to heparan sulfate proteoglycans (1), the extracellular matrix protein agrin (2), and several chondroitin sulfate proteoglycans that include neurocan and phosphocan (3). There are three main forms of human NCAM-1 that arise by alternate splicing. These are designated NCAM-120/NCAM-1 (761 amino acids [aa]), NCAM‑140 (848 aa), and NCAM-180 (1120 aa). NCAM-120 is GPI-linked, while NCAM‑140 and NCAM-180 are type I transmembrane glycoproteins (4 - 6). Additional alternate splicing adds considerable diversity to all three forms, and extracellular proteolytic processing is possible for NCAM-180 (7 ‑ 8). NCAM-1 is synthesized as a 761 aa preproprecursor that contains a 19 aa signal sequence, a 722 aa GPI-linked mature region, and a 20 aa C-terminal prosegment (4). The molecule contains five C-2 type Ig-like domains and two fibronectin type-III domains. Human to mouse, NCAM-1 is 93% aa identical. NCAM-1 appears to be highly sialylated. The polysialyation of NCAM-1 reduces its adhesive property and increases its neurite outgrowth promoting features (9). NCAM-1 in the adult brain shows a decline of sialylation relative to earlier developmental periods. In regions that retain a high degree of neuronal plasticity, however, the adult brain continues to express polysialylation-NCAM-1, suggesting sialylation of NCAM-1 is involved in regenerative processes and synaptic plasticity (10 - 13).

References

Burg, M.A. et al. (1995) J. Neurosci. Res. 41:49.
Storms, S.D. and U. Rutishauser (1998) J Biol. Chem. 273:27124.
Margolis, R.K. et al. (1996) Perspect. Dev. Neurobiol. 3:273.
Dickson, G. et al. (1987) Cell 50:1119.
Lanier, L.L. et al. (1991) J. Immunol. 146:4421.
Hemperly, J.J. et al. (1990) J. Mol. Neurosci. 2:71.
Rutishauser, U.and C. Goridis (1986) Trends Genet. 2:72.
Vawter, M.P. et al. (2001) Exp. Neurol. 172:29.
Rutihauser, U. (1990) Adv. Exp. Med. Biol. 265:179.
Becker, C.G. et al. (1996) J. Neurosci. Res. 45:143.
Doherty, P. et al. (1995) J. Neurobiol. 26:437.
Eckardt, M. et al. (2000) J. Neurosci. 20:5234.
Muller, D. et al. (1996) Neuron 17:413.

Long Name

Neural Cell Adhesion Molecule

Entrez Gene IDs

4684 (Human); 17967 (Mouse); 24586 (Rat)

Alternate Names

CD56 / NCAM-1; CD56 antigen; CD56; MSK39; NCAM1; N-CAM-1; NCAM-1; NCAMantigen recognized by monoclonal 5.1H11; neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM