Human Napsin A Antibody

Catalog #: MAB8489 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8489
MAB8489-SP

Detection of Human Napsin A by Western Blot.

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All Napsin A Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Napsin A Antibody Summary

Species Reactivity

Human

Specificity

Detects human Napsin A in direct ELISAs and Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1265A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Napsin A
Thr25-Gly420
Accession # O96009

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Simple Western

5 µg/mL

See below

Immunohistochemistry

1-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Napsin A by Western Blot. Western blot shows lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Napsin A Monoclonal Antibody (Catalog # MAB8489) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Napsin A at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Napsin A in Human Kidney. Napsin A was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Napsin A Monoclonal Antibody (Catalog # MAB8489) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western

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Detection of Human Napsin A by Simple Western^TM. Simple Western lane view shows lysates of human lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Napsin A at approximately 46 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Napsin A Monoclonal Antibody (Catalog # MAB8489). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Napsin A

Novel Aspartic Proteinase of the Pepsin Family or Napsin A is an aspartic proteinase that belongs to the peptidase A1 family and plays a role in pneumocyte surfactant processing. It consists of a 24-residue signal peptide, a 40-amino acid propart, the mature enzyme of 336 amino acids, and a C-terminal extension of 18 residues. Highest levels of Napsin A have been detected in adult lung (type II pneumocytes), fetal lung, and kidney tissues. Napsin A is also expressed at lower levels in adult spleen and at very low levels in peripheral blood leukocytes. Human napsin A shares 72.6% sequence identity with the mouse homolog.

Entrez Gene IDs

9476 (Human); 16541 (Mouse); 60575 (Rat)

Alternate Names

Asp 4; ASP4; Aspartyl protease 4; EC 3.4.23; EC 3.4.23.-; EC 3.4.23.15; EC 3.4.23.3; EC 3.4.23.5; KAP; Kdap; NAP1napsin-A; NAPA; NAPApronapsin A; NAPSA; napsin A aspartic peptidase; Napsin A; Napsin-1; SNAPA; TA01/TA02