Human N-Terminal Pro-BNP Antibody

Catalog # Availability Size / Price Qty
MAB9538-100
MAB9538-SP
Human NT Pro-BNP ELISA Standard Curve.
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Human N-Terminal Pro-BNP Antibody Summary

Species Reactivity
Human
Specificity
Detects human N-Terminal Pro-BNP in direct ELISAs.
Source
Monoclonal Mouse IgG2b Clone # 370129
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA captureantibody when paired with Rabbit Anti-Human N-terminal Pro-BNP Monoclonal Antibody (Catalog # MAB36042).

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human N-Terminal Pro- BNP Monoclonal Antibody (Catalog # MAB9769).

This product is intended for assay development onvarious assay platforms requiring antibody pairs. We recommend the Human NT Pro-BNP DuoSet ELISA Kit (Catalog # DY3604-05)for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Human NT Pro-BNP ELISA Standard Curve. Recombinant human Pro-BNP protein was serially diluted 2-fold and captured by Mouse Anti-Human N-Terminal Pro-BNP Monoclonal Antibody (Catalog # MAB9538) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human N-Terminal Pro-BNP Monoclonal Antibody (Catalog # MAB36042) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Human NT Pro-BNP ELISA Standard Curve. Recombinant Human Pro-BNP protein was serially diluted 2-fold and captured by Mouse Anti-Human N-Terminal Pro- BNP Monoclonal Antibody (Catalog # MAB9769) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human N-Terminal Pro BNP Monoclonal Antibody (Catalog # MAB9538) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: BNP

Brain-type Natriuretic Peptide (BNP) is a nonglycosylated peptide that is produced predominantly by ventricular myocytes and belongs to the natriuretic peptide family. Proteolytic cleavage of the 12 kDa BNP precursor gives rise to N-terminal Pro‑BNP (NT-proBNP) and mature BNP. Plasma NT-proBNP is a marker for congestive heart failure, while mature BNP (aa 103‑134) promotes vasodilation and fluid and sodium excretion. Human BNP precursor shares 29% and 51% aa sequence identity with mouse and porcine BNP precursor, respectively.

Long Name
Brain Natriuretic Peptide
Entrez Gene IDs
4879 (Human); 18158 (Mouse); 25105 (Rat)
Alternate Names
BNF; BNP; brain type natriuretic peptide; Gamma-brain natriuretic peptide; natriuretic peptide B; natriuretic peptide precursor B; natriuretic peptides B; natriuretic protein; NPPB

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